| CAS Number | 1051375-16-6 |
|---|---|
| Molecular Formula | C20H19F2N3O5 |
| Molecular Weight | 419.4 |
| InChI Key | RHWKPHLQXYSBKR-BMIGLBTASA-N |
| LogP | 2.4 |
| Synonyms |
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Applications:
HPLC Method for Analysis of Dolutegravir and Dolutegravir Intermediate-1 on Primesep B Column
January 10, 2025
HPLC Method for Dolutegravir, Dolutegravir Intermediate-1 on Primesep B by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Dolutegravir, Dolutegravir Intermediate-1
Dolutegravir Intermediate-1 is a key chemical precursor used in the synthesis of Dolutegravir, an antiretroviral drug for the treatment of HIV infection. This intermediate plays a crucial role in building the core structure of Dolutegravir, contributing to its integrase strand transfer inhibitor (INSTI) activity, which prevents the virus from integrating its genetic material into the host cell’s DNA. Its synthesis requires precise chemical processes to ensure high purity and yield, as it directly impacts the efficacy and safety of the final pharmaceutical product.
Dolutegravir, Dolutegravir Intermediate-1 can be retained, and analyzed using a Primesep B mixed-mode stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water, acetonitrile (MeCN), and phosphoric acid as a buffer. Detection is carried out using UV.
| Column | Primesep B, 4.6 x 150 mm, 5 µm, 100 A |
| Mobile Phase | Gradient MeCN 30% – 90%, 10 min |
| Buffer | H3PO4 – 0.1% |
| Flow Rate | 1 ml/min |
| Detection | UV 270 nm |
| Class of Compounds | Antiretroviral Drug |
| Analyzing Compounds | Dolutegravir, Dolutegravir Intermediate-1 |
Application Column
Primesep B
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Dolutegravir Intermediate-1
