Access the UV-Vis Spectrum SIELC Library

If you are looking for optimized HPLC method to analyze Dolutegravir check our HPLC Applications library

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.

HPLC Method for Dolutegravir, Dolutegravir Intermediate-1 on Primesep B by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Dolutegravir, Dolutegravir Intermediate-1

Dolutegravir Intermediate-1 is a key chemical precursor used in the synthesis of Dolutegravir, an antiretroviral drug for the treatment of HIV infection. This intermediate plays a crucial role in building the core structure of Dolutegravir, contributing to its integrase strand transfer inhibitor (INSTI) activity, which prevents the virus from integrating its genetic material into the host cell’s DNA. Its synthesis requires precise chemical processes to ensure high purity and yield, as it directly impacts the efficacy and safety of the final pharmaceutical product.

Dolutegravir, Dolutegravir Intermediate-1 can be retained, and analyzed using a Primesep B mixed-mode stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water, acetonitrile (MeCN), and phosphoric acid as a buffer. Detection is carried out using UV.