Cyanoacetic acid

Cyanoacetic acid structural formula

CAS Number372-09-8
Molecular FormulaC3H3NO2
Molecular Weight85.062
InChI KeyMLIREBYILWEBDM-UHFFFAOYSA-N
LogP-0.760
Synonyms
  • Cyanoacetic acid
  • Acetic acid, 2-cyano-
  • 372-09-8
  • 4-02-00-01888
  • Acetic acid, cyano-
  • 2-Cyanoacetic acid
  • Acetic acid, 2-cyano-
  • ACETIC ACID, CYANO
  • Acide cyanoacetique
  • acido cianoacetico
  • CYANESSIGSAEURE
  • Cyanessigsaure
  • Cyanoethanoic acid
  • Malonic mononitrile
  • Monocyanoacetic acid
  • NSC 5571
  • BRN 0506325
  • EINECS 206-743-9
  • Acide cyanacetique
  • Kyselina kyanoctova
  • UNII-QZT550H2Y9
  • Malonic acid mononitrile

Applications:

HPLC Determination of Cyanoacetic Acid on Primesep SB Column

October 1, 2019

HPLC Method for Cyanoacetic acid on Primesep SB by SIELC Technologies

HPLC Determination of Cyanoacetic Acid on Primesep SB Column_1187

High Performance Liquid Chromatography (HPLC) Method for Analysis of Cyanoacetic acid 

Cyanoacetic Acid is an organic acid with the chemical formula C3H3NO2. It is used in a variety of syntheses as an intermediate including but not limited to vitamin B6, caffeine, herbicides, pesticides, dyes, and polymers. When heated up, it undergoes decarboxylation to produce acetonitrile.

Cyanoacetic acid can be retained and analyzed using the Primesep SB stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.

Condition
Column Primesep SB, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN/H2O – 20/80%
Buffer H2SO4 – 0.05%
Flow Rate 1.0 ml/min
Detection UV, 200 nm

Description
Class of Compounds Acid, Hydrophilic, Ionizable
Analyzing Compounds Cyanoacetic acid

Application Column

Primesep SB

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

Add to cart
Application Analytes:
Cyanoacetic acid
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Cyanoacetic acid on Newcrom R1 HPLC column

February 16, 2018
Separation of Cyanoacetic acid on Newcrom C18 HPLC column

Cyanoacetic acid can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Cyanoacetic acid
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us before ordering the column as there may be a more suitable column alternative by either e-mail: support@sielc.com or by phone: 847-229-2629.