Histamine

CAS Number	51-45-6
Molecular Formula	C5H9N3
Molecular Weight	111.149
InChI Key	NTYJJOPFIAHURM-UHFFFAOYSA-N
LogP	-0.700
Synonyms	Histamine 2-(1H-Imidazol-5-yl)ethan-1-amine 1H-Imidazole-5-ethanamine 51-45-6 1H-Imidazole-4-ethanamine 1H-Imidazole-5-ethanamine 2-(1H-Imidazol-4-yl)ethanamine 2-(1H-Imidazol-4-yl)ethylamine 2-(1H-Imidazol-5-yl)ethanamine 2-(1H-Imidazol-5-yl)ethylamine 2-(3H-Imidazol-4-yl)ethylamine 2-(4-Imidazolyl)ethanamine 2-(4-Imidazolyl)ethylamine 2-imidazol-4-iletilamina 2-Imidazol-4-ylethylamin 2-Imidazol-4-ylethylamine 2-imidazole-4-ylethylamine 4-(2-Aminoethyl)-1H-imidazole 4-(2-Aminoethyl)imidazole 5-Imidazoleethylamine Ergamine Ergotidine Imidazole-4-ethylamine NSC 33792 β-Imidazolyl-4-ethylamine EINECS 200-100-6 Ethylamine, 2-imidazol-4-yl- Imidazole, 4-(2-aminoethyl)- Istamina UNII-820484N8I3 2-(1H-imidazol-4-yl)ethan-1-amine 2-Imidazol-4-yl-Ethylamine 4-Imidazoleethylamine Eramin Free histamine HSM L-Histamin base L-Histamine Theramine b-Imidazolyl-4-ethylamine beta-Aminoethylglyoxaline beta-Aminoethylimidazole beta-Aminothethylglyoxaline beta-Imidazolyl-4-ethylamine 64422-25-9 924364-91-0

Applications:

Uv-Vis Spectrum of Histamine

January 19, 2026

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Access the UV-Vis Spectrum SIELC Library

If you are looking for optimized HPLC method to analyze Histamine check our HPLC Applications library

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.

Histamine

SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Determination of Histamine on BIST A+ Column by SIELC Technologies

October 18, 2022

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HPLC Method for Determination of Histamine on BIST A+ Column by SIELC Technologies

Histamine dihydrochloride, also known as Ceplene, is a medication with the chemical formula C₅H₁₁Cl₂N₃. It is used primarily to prevent a relapse of acute myeloid leukemia. Other pharmaceutical applications include as a topical analgesic to treat arthritis, aches, bruises, sprains, and strains. Brand names it is sold under include Australian Dream and Alo.

Histamine dihydrochloride can be detected in the low UV regime. Using a BIST A+ column and a mobile phase consisting of water and Acetonitrile (MeCN) with an ammonium formate (AmFm) buffer, Histamine chloride can be retained, separated, and analyzed. This analysis method can be detected and is compatible with Mass Spectrometry (MS), ELSD, and CAD.

Condition

Column	BIST A+, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase	MeCN – 70%
Buffer	Ammonium Formate pH 3.0 – 20 mM
Flow Rate	1.0 ml/min
Detection	ELSD, 50C

Description

Class of Compounds	Drug
Analyzing Compounds	Histamine

Application Column

BIST A+

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Histamine

ELSD Detection

HPLC Separation of Histamine and Phenethylamine on Primesep C Column

August 7, 2010

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Histamine is a basic compound that acts like neurotransmitter. Phenethylamine is a natural amine alkaloid psychoactive compound with stimulant effects. It forms by enzymatic decarboxylation of phenylalanine, and also acts as neurotransmitter. Both compounds are separated in one HPLC run on Primesep C and Primesep 200 columns by reversed-phase cation-exchange mechanism. Primesep C provides shorter retention time and histamine and phenethylamine can be separated within 5 minutes. Primesep C and Primesep 200 columns do not require ion-pairing reagent as it is attached to the surface of Primesep silica gel. Method can be used for analysis of these two compounds in plasma, blood and urine with UV, ELSD, CAD or LC/MS detection.