HPLC Method for Determination of Histamine on BIST A+ Column by SIELC Technologies

HPLC Method for Determination of Histamine on BIST A+ Column by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode

HPLC Method for Determination of Histamine on BIST A+ Column
HPLC Method for Determination of Histamine on BIST A+ Column by SIELC Technolog

High Performance Liquid Chromatography (HPLC) Method for Analyses is of Histamine BIST A+ Column by SIELC Technologies

Histamine dihydrochloride, also known as Ceplene, is used to prevent a relapse of acute myeloid leukemia. Other pharmaceutical applications include as a topical analgesic to treat arthritis, aches, bruises, sprains, and strains. Brand names it is sold under include Australian Dream and Alo. Histamine dihydrochloride can be detected in the low UV regime. Using a BIST A+ column and a mobile phase consisting of water and Acetonitrile (MeCN) with an ammonium formate (AmFm) buffer, Histamine chloride can be retained, separated, and analyzed. This analysis method can be detected and is compatible with Mass Spectrometry (MS), ELSD, and CAD. 

Condition

ColumnBIST A+, 4.6x150 mm, 5µm, 100A
Mobile PhaseMeCN - 70%
BufferAmmonium Formate pH 3.0 - 20 mM
Flow Rate1.0 ml/min
DetectionELSD, 50C

Description

Class of CompoundsDrug
Analyzing CompoundsHistamine
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
Application Analytes:
Histamine