Neomycin

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CAS Number119-04-0
Molecular FormulaC23H46N6O13
Molecular Weight614.6
InChI KeyPGBHMTALBVVCIT-VCIWKGPPSA-N
LogP-9
Synonyms
  • neomycin
  • Fradiomycin
  • Neomas
  • Nivemycin
  • Myacyne
  • Neolate
  • Caswell No. 595
  • Vonamycin powder V
  • Neomin
  • Neomicina
  • Neomycine
  • Neomycinum
  • PIMAVECORT
  • 1404-04-2
  • (2R,3S,4R,5R,6R)-5-amino-2-(aminomethyl)-6-[(1R,2R,3S,4R,6S)-4,6-diamino-2-[(2S,3R,4S,5R)-4-[(2R,3R,4R,5S,6S)-3-amino-6-(aminomethyl)-4,5-dihydroxyoxan-2-yl]oxy-3-hydroxy-5-(hydroxymethyl)oxolan-2-yl]oxy-3-hydroxycyclohexyl]oxyoxane-3,4-diol
  • I16QD7X297
  • VONAMYCIN
  • Kaomycine
  • Neomycin Complex
  • (2R,3S,4R,5R,6R)-5-amino-2-(aminomethyl)-6-((1R,2R,3S,4R,6S)-4,6-diamino-2-((2S,3R,4S,5R)-4-((2R,3R,4R,5S,6S)-3-amino-6-(aminomethyl)-4,5-dihydroxyoxan-2-yl)oxy-3-hydroxy-5-(hydroxymethyl)oxolan-2-yl)oxy-3-hydroxycyclohexyl)oxyoxane-3,4-diol
  • RefChem:55694
  • CHEBI:7507
  • DTXSID9041073
  • A01AB08
  • A07AA01
  • B05CA09
  • D06AX04
  • J01GB05
  • R02AB01
  • S01AA03
  • S02AA07
  • S03AA01
  • 215-766-3
  • Framycetin
  • NEOMYCIN B
  • 119-04-0
  • Soframycin
  • Actilin
  • Framygen
  • Fradiomycin B
  • Enterfram
  • Framicetina
  • Framycetine
  • Fradiomycinum
  • Framycetinum
  • Antibiotic 10676
  • Neobrettin
  • Actiline
  • CCRIS 5462
  • Framidal
  • Framycin
  • Francetin
  • Dekamycin iii
  • HSDB 3242
  • Soframycine
  • Framycetin (INN)
  • Neomycin B Hexaacetate
  • ANTIBIOTIQUE EF 185
  • Neomcin
  • 4BOC774388
  • (1R,2R,3S,4R,6S)-4,6-diamino-2-{[3-O-(2,6-diamino-2,6-dideoxy-beta-L-idopyranosyl)-beta-D-ribofuranosyl]oxy}-3-hydroxycyclohexyl 2,6-diamino-2,6-dideoxy-alpha-D-glucopyranoside
  • Neomicina [DCIT]
  • Framycetinum [INN-Latin]
  • Bycomycin
  • FRAMYCETIN [INN]
  • Neomycine [INN-French]
  • Neomycinum [INN-Latin]
  • Framycetine [INN-French]
  • NMY
  • Framicetina [INN-Spanish]
  • Framycetin [INN:BAN:DCF]
  • Jernadex
  • Neomycin Trisulfate Hydrate Deuterated
  • Fradiomycin Trisulfate Hydrate deuterated
  • (2R,3S,4R,5R,6R)-5-amino-2-(aminomethyl)-6-[(1R,2R,3S,4R,6S)-4,6-diamino-2-[(2S,3R,4S,5R)-4-[(2R,3R,4R,5S,6S)-3-amino-6-(aminomethyl)-4,5-dihydroxy-tetrahydropyran-2-yl]oxy-3-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl]oxy-3-hydroxy-cyclohexoxy]tetrahydropyran-3,4-diol
  • 11004-65-2
  • Fradiomycin B;Neomycin B
  • Neomycin [INN:BAN]
  • C23H46N6O13
  • EINECS 204-292-2
  • EINECS 215-766-3
  • EPA Pesticide Chemical Code 006303
  • BRN 0101621
  • Mycerin
  • UNII-I16QD7X297
  • Actilin;
  • UNII-4BOC774388
  • Neomycin B;
  • Antibiotic 956
  • Neomycin B Acetate
  • Antibiotic produced by Streptomyces decaris. Neomycin B
  • NEOMYCIN B [MI]
  • Prestwick3_000158
  • Neomycin B; Fradiomycin B
  • SCHEMBL3279
  • FRAMYCETIN [WHO-DD]
  • BSPBio_000296
  • GTPL709
  • NEOMYCIN B [USP-RS]
  • 4-18-00-07476 (Beilstein Handbook Reference)
  • BPBio1_000326
  • CHEBI:7508
  • CHEMBL184618
  • orb1707294
  • DTXSID2023359
  • y)tetrahydro-2H-pyran-3,4-diol
  • GLXC-10574
  • HMS2089P15
  • EBC-27391
  • MFCD09788228
  • AKOS024284361
  • CS-6390
  • DB00452
  • NCGC00179612-01
  • D-Streptamine, O-2,6-diamino-2,6-dideoxy-.beta.-L-idopyranosyl-(1.->3)-O-.beta.-D-ribofuranosyl-(1->5)]-O-[2,6-diamino-2,6-dideoxy-.alpha.-D-glucopyranosyl-(1->4)]-2-deoxy
  • D-Streptamine, O-2,6-diamino-2,6-dideoxy-alpha-D-glucopyranosyl-(1-4)-O-(O-2,6-diamino-2,6-dideoxy-beta-L-idopyranosyl-(1-3)-beta-D-ribofuranosyl-(1-5))-2-deoxy-
  • DA-63612
  • FN175622
  • HY-17624
  • ST075177
  • AB00443887
  • NS00072944
  • C01737
  • D05140
  • AB00443887-03
  • EN300-7480789
  • MYCIFRADIN; NEOMAS; PIMAVECORT; VONAMYCIN
  • ANTIBIOTIC PRODUCED BY STREPTOMYCES DECARIS
  • Q4492348
  • BRD-K71013094-001-02-5
  • BRD-K71013094-065-01-2
  • BRD-K71013094-326-01-8
  • (2R,3S,4R,5R,6R)-5-amino-2-(aminomethyl)-6-{[(1R,2R,3S,4R,6S)-4,6-diamino-2-{[(2S,3R,4S,5R)-4-{[(2R,3R,4R,5S,6S)-3-amino-6-(aminomethyl)-4,5-dihydroxyoxan-2-yl]oxy}-3-hydroxy-5-(hydroxymethyl)oxolan-2-yl]oxy}-3-hydroxycyclohexyl]oxy}oxane-3,4-diol
  • (2S,3S,4R,5R,6R)-5-Amino-2-(aminomethyl)-6-(((2R,3S,4R,5S)-5-(((1R,2R,3S,5R,6S)-3,5-diamino-2-(((2R,3R,4R,5S,6R)-3-amino-6-(aminomethyl)-4,5-dihydroxytetrahydro-2H-pyran-2-yl)oxy)-6-hydroxycyclohexyl)oxy)-4-hydroxy-2-(hydroxymethyl)tetrahydrofuran-3-yl)oxy)tetrahydro-2H-pyran-3,4-diol
  • (2S,3S,5R,6R)-5-amino-2-(aminomethyl)-6-(((2R,3S,4R,5S)-5-(((1R,2R,3S,5R,6S)-3,5-diamino-2-(((2R,3R,4R,5S,6R)-3-amino-6-(aminomethyl)-4,5-dihydroxytetrahydro-2H-pyran-2-yl)oxy)-6-hydroxycyclohexyl)oxy)-4-hydroxy-2-(hydroxymethyl)tetrahydrofuran-3-yl)oxy)tetrahydro-2H-pyran-3,4-diol
  • 6-(2-{(4S,2R,3R,5R)-4-[(5S,6S,2R,3R,4R)-3-amino-6-(aminomethyl)-4,5-dihydroxy( 2H-3,4,5,6-tetrahydropyran-2-yloxy)]-3-hydroxy-5-(hydroxymethyl)oxolan-2-yloxy }(3S,6S,1R,2R,4R)-4,6-diamino-3-hydroxycyc
  • 6-(2-{(4S,2R,3R,5R)-4-[(5S,6S,2R,3R,4R)-3-amino-6-(aminomethyl)-4,5-dihydroxy( 2H-3,4,5,6-tetrahydropyran-2-yloxy)]-3-hydroxy-5-(hydroxymethyl)oxolan-2-yloxy }(3S,6S,1R,2R,4R)-4,6-diamino-3-hydroxycyclohexyloxy)(3S,2R,4R,5R,6R)-5-amino- 2-(aminomethyl)-2H
  • D-STREPTAMINE, O-2,6-DIAMINO-2,6-DIDEOXY-.ALPHA.-D-GLUCOPYRANOSYL-(1->4)-O-(O-2,6-DIAMINO-2,6-DIDEOXY-.BETA.-L-IDOPYRANOSYL-(1->3)-.BETA.-D-RIBOFURANOSYL-(1->5))-2-DEOXY-
  • D-STREPTAMINE, O-2,6-DIAMINO-2,6-DIDEOXY-.BETA.-L-IDOPYRANOSYL-(1->3)-O-.BETA.-D-RIBOFURANOSYL-(1->5)-O-(2,6-DIAMINO-2,6-DIDEOXY-.ALPHA.-D-GLUCOPYRANOSYL-(1->4))-2-DEOXY-
  • lohexyloxy)(3S,2R,4R,5R,6R)-5-amino- 2-(aminomethyl)-2H-3,4,5,6-tetrahydropyran-3,4-diol

Applications:

HPLC MS Method for Analysis of Neomycin on Chromni Column

September 10, 2026

HPLC Method for Analysis of Neomycin on Chromni Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Neomycin.

Neomycin is the an aminoglycoside antibiotic with the chemical formula C23H46N6O13. It is primarily used in topical preparations. It is is often combined with other compounds to create widely used medications. Polymyxin B, which combines neomycin, polymyxin B, and hydrocortisone is one of the most prescribed medications in the United States. It works through binding to the 30S subunit of the prokaryotic ribosome, therefore inhibiting prokaryotic translation of mRNA. You can find detailed UV spectra of Neomycin and information about its various lambda maxima by visiting the following link.

Neomycin can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN). Detection is performed using LC MS.

Condition

ColumnChromni, 3.2 x 100 mm, 3 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O
BufferAmmonium Formate
Flow Rate0.5 ml/min
DetectionLC MS ESI SIM 615(M+H)+
LOD*20 ppm
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Description

Class of CompoundsAntibiotic
Analyzing CompoundsNeomycin

Application Column

Chromni

Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Neomycin
Application Detection:
LC MS Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Uv-Vis Spectrum of Neomycin

January 23, 2026
Access the UV-Vis Spectrum SIELC Library
UV-Vis Spectrum of Neomycin.

If you are looking for optimized HPLC method to analyze Neomycin check our HPLC Applications library

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.

 

 

Application Analytes:
Neomycin
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.