C.I. Acid Blue 74

C.I. Acid Blue 74 structural formula

CAS Number860-22-0
Molecular FormulaC16H8N2Na2O8S2
Molecular Weight466.350
InChI KeyKHLVKKOJDHCJMG-QDBORUFSSA-L
LogP0.130
Synonyms
  • C.I. Acid Blue 74
  • Disodium (2E)-3-oxo-2-(3-oxo-5-sulfonato-1,3-dihydro-2H-indol-2-ylidene)-2,3-dihydro-1H-indole-5-sulfonate
  • 1H-Indole-5-sulfonic acid, 2-(1,3-dihydro-3-oxo-5-sulfo-2H-indol-2-ylidene)-2,3-dihydro-3-oxo-, sodium salt, (2E)- (1:2)
  • 860-22-0
  • Indigo carmine
  • FD & C Blue No. 2
  • Disodium 3-oxo-2-(3-oxo-5-sulfonato-1,3-dihydro-2H-indol-2-ylidene)-2,3-dihydro-1H-indole-5-sulfonate
  • 12070 Blue
  • 1311 Blue
  • Aniline Carmine Powder
  • Basovit Blue 665E
  • Blue 2
  • Blue No. 2
  • C.I. 73015
  • C.I. 75781
  • Carmine Blue
  • Carmine indigo
  • Cogilor Blue 511.11
  • FD & C Blue 2
  • FD & C Blue No 2-307045
  • FD and C Blue 2
  • FD&C Blue No. 2-37006
  • Food Blue 1
  • Food Blue 2
  • Food Blue No. 1
  • Indigo Carmine Powder
  • Indigo carmine 85E132
  • Indigo carmine E 132
  • Indigotin (solubilized)
  • Japan Blue 2
  • Japan Food Blue No. 2
  • Usacert FD & C Blue No 2-310118
  • Usacert FD and C Blue No. 2
  • WAS 35
  • Water Blue 177557
  • Water Blue 177558
  • C.I. 73015, C.I. 75781
  • disodium 3,3'-dioxo-2,2'-biindolylidene-5,5'-disulfonate
  • 1H-Indole-5-sulfonic acid, 2-(1,3-dihydro-3-oxo-5-sulfo-2h-indol-2-ylidene)-2,3-dihydro-3-oxo-, disodium salt
  • 1h-Indol-5-acido sulfonico, 2-(1,3-dihidro-3-oxo-5-sulfo-2H-indol-2-iliden) -2,3-dihidro-3-oxo-, sal de sodio (1:2)
  • [?2,2'-Biindoline]-5,5'-disulfonic acid, 3,3'-dioxo-, disodium salt
  • 1H-Indole-5-sulfonic acid, 2-(1,3-dihydro-3-oxo-5-sulfo-2H-indol-2-ylidene)-2,3-dihydro-3-oxo, disodium salt
  • 1H-Indole-5-sulfonic acid, 2-(1,3-dihydro-3-oxo-5-sulfo-2H-indol-2-ylidene)-2,3-dihydro-3-oxo-, sodium salt (1:2)
  • 2-(1,3-dihydro-3-oxo-5-sulfo-2H-indol-2-ylidene)-2,3-dihydro-3-oxo-1H-Indole-5-sulfonic acid disodium salt
  • 5,5'-(2-(1,3-dihidro-3-oxo-2H-indazol-2-ilideno)-1,2-dihidro-3H-indol-3-ona)disulfonato de disodio
  • 5,5'-(2-(1,3-Dihydro-3-oxo-2H-indazol-2-ylidene)-1,2-dihydro-3H-indol-3-one)disulfonate de disodium
  • 5,5'-(2-(1,3-dihydro-3-oxo-2H-indazole-2-ylidene)-1,2-dihydro-3H-indole-3-one)disulfonate de disodium
  • 5,5'-Indigodisulfonic acid disodium salt
  • Ariavit Indigo Carmine
  • Atul Indigo Carmine
  • Bucacid Indigotine B
  • Canacert Indigo Carmine
  • Dinatrium-5,5'-(2-(1,3-dihydro-3-oxo-2H-indazol-2-yliden)-1,2-dihydro-3H-indol-3-on)disulfonat
  • disodium 5,5'-(2-(1,3-dihydro-3-oxo-2H-indazol-2-ylidene)-1,2-dihydro-3H-indol-3-one)disulphonate
  • Disodium 5,5'-indigodisulfonate
  • Disodium 5,5'-indigotin disulfonate
  • Dolkwal Indigo Carmine
  • Eurocert Indigo Carmine 311811
  • HD Indigo Carmine
  • HD Indigo Carmine Supra
  • Hexacol Indigo Carmine Supra
  • Indigo Carmine 307019
  • Indigo Carmine 36009
  • Indigo Carmine 37006
  • Indigo Carmine A
  • Indigo Carmine AC
  • Indigo Carmine BP
  • Indigo carmine NB
  • Indigo Carmine X
  • Indigotine B
  • Indigotine Carmine
  • Indigotine disodium salt
  • Indigotine I
  • Indigotine IA
  • Indigotine Lake
  • Indigotine N
  • Indocarmine F
  • Maple Indigo Carmine
  • Mitsui Indigo Carmine
  • Necol Indigo Carmine
  • Neelicol Indigo Carmine
  • San-ei Indigo Carmine
  • Sepisperse Dry 1003
  • Sodium 5,5'-indigodisulfonate
  • Sodium 5,5'-indigotindisulfonate
  • Soluble indigo
  • Soluble indigo blue
  • Indigotin
  • L Blue Z 5010
  • A.F. Blue No. 2
  • Acid Blue W
  • Acid Blue 74
  • Acid Leather Blue IC
  • Airedale Blue IN
  • Amacid Brilliant Blue
  • (delta2,2'-Biindoline)-5,5'-disulfonic acid, 3,3'-dioxo-, disodium salt
  • Cilefa Blue R
  • Disodium 2-(1,3-dihydro-3-oxo-5-sulfo-2H-indol-2-ylidene)-2,3-dihydro-3-oxo-1H-indole-5-sulfonate
  • Disodium salt of 1-indigotin-S,S'-disulfonic acid
  • Disodium salt of 1-indigotin-S,S'-disulphonic acid
  • Edicol Supra Blue X
  • EINECS 212-728-8
  • Food Blue No. 2
  • Grape Blue A
  • Grape Blue A Geigy
  • Hexacert Blue No. 2
  • Indigo Carmine Conc. FQ
  • Indigo Carmine disodium salt
  • Indigo extract
  • Indigotin I
  • Indigotine
  • Indigotine Blue LZ
  • Indigotine Conc. Powder; Indigotine Extra Pure A
  • Indigotine Extra Pure A
  • Intense Blue
  • Murabba
  • NSC 8646
  • Sachsischblau
  • Sumitomo Wool Blue SBC
  • Usacert Blue No. 2
  • Dinatrium 3,3'-dioxo-(delta2,2'-biindolin)-5,5'-disulfonat
  • Dinatrium indigolin-5,5'-disulfonat
  • Indigocarmin
  • Indigo-karmin
  • Modr Kysela 74
  • Modr Pigment 63
  • Modr Potravinarska 1
  • Schultz Nr. 1309
  • UNII-D3741U8K7L
  • FD &C Blue No. 2
  • C.I. Food Blue 1
  • C.I. Food Blue 1, disodium salt
  • C.I. Natural Blue 2
  • Disodium 3,3'-dioxo-(delta2,2'-biindoline)-5,5'-disulfonate
  • Disodium 5,5'-disulfoindigo
  • Disodium 5,5'-indigotin disulfonate
  • Disodium indigo-5,5-disulfonate
  • E 132
  • FD and C Blue No. 2
  • FD&C Blue No. 2
  • Indigocarmine
  • Indigotin-5,5'-disulfonic acid disodium salt
  • Indigotindisulfonate sodium
  • Sodium 5,5'-indigotindisulfonate
  • Sodium indigo-5,5'-bisulfonate
  • Sodium indigotindisulfonate

Applications:

Uv-Vis Spectrum of Indigo Carmine

April 7, 2026

Access the UV-Vis Spectrum SIELC Library

If you are looking for optimized HPLC method to analyze C.I. Acid Blue 74 check our HPLC Applications library

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase.  The compound was dissolved with water. This analysis was done with the mobile phase made of 20% acetonitrile and 80% water.

 

 

Application Analytes:
C.I. Acid Blue 74
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of C.I. Acid Blue 74 on Newcrom R1 HPLC column

February 16, 2018
Separation of C.I. Acid Blue 74 on Newcrom C18 HPLC column

C.I. Acid Blue 74 can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
C.I. Acid Blue 74
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us before ordering the column as there may be a more suitable column alternative by either e-mail: support@sielc.com or by phone: 847-229-2629.