Brilliant Blue R

CAS Number6104-59-2
Molecular FormulaC45H44N3NaO7S2
Molecular Weight826.0
InChI KeyNKLPQNGYXWVELD-UHFFFAOYSA-M
Synonyms
  • 6104-59-2
  • Brilliant Blue R
  • COOMASSIE BRILLIANT BLUE R
  • C.I. Acid Blue 83
  • TKL1K0PT9A
  • Eriosin Brilliant Cyanine 6B
  • Coomassie blue R 250
  • EINECS 228-060-5
  • DTXSID80910613
  • COOMASSIE BRILLIANT BLUE R [MI]
  • Benzenemethanaminium, N-(4-((4-((4-ethoxyphenyl)amino)phenyl)(4-(ethyl((3-sulfophenyl)methyl)amino)phenyl)methylene)-2,5-cyclohexadien-1-ylidene)-N-ethyl-3-sulfo-, inner salt, sodium salt (1:1)
  • Benzenemethanaminium, N-(4-((4-((4-ethoxyphenyl)amino)phenyl)(4-(ethyl((3-sulfophenyl)methyl)amino)phenyl)methylene)-2,5-cyclohexadien-1-ylidene)-N-ethyl-3-sulfo-, hydroxide, inner salt, monosodium salt
  • Benzenemethanaminium, N-(4-((4-((4-ethoxyphenyl)amino)phenyl)(4-(ethyl((3-sulfophenyl)methyl)amino)phenyl)methylene)-2,5-cyclohexadien-1-ylidene)-N-ethyl-3-sulfo-, inner salt, monosodium salt
  • Benzenemethanaminium, N-[4-[[4-[(4-ethoxyphenyl)amino]phenyl][4-[ethyl[(3-sulfophenyl)methyl]amino]phenyl]methylene]-2,5-cyclohexadien-1-ylidene]-N-ethyl-3-sulfo-, hydroxide, inner salt, monosodium salt
  • Benzenemethanaminium, N-[4-[[4-[(4-ethoxyphenyl)amino]phenyl][4-[ethyl[(3-sulfophenyl)methyl]amino]phenyl]methylene]-2,5-cyclohexadien-1-ylidene]-N-ethyl-3-sulfo-, inner salt, monosodium salt
  • Hydrogen (4-(4-(p-ethoxyanilino)-4'-(ethyl(m-sulphonatobenzyl)amino)benzhydrylene)cyclohexa-2,5-dien-1-ylidene)(ethyl)(m-sulphonatobenzyl)ammonium, monosodium salt
  • Hydrogen [4-[4-(p-ethoxyanilino)-4'-[ethyl(m-sulphonatobenzyl)amino]benzhydrylene]cyclohexa-2,5-dien-1-ylidene](ethyl)(m-sulphonatobenzyl)ammonium, monosodium salt
  • Benzenemethanaminium, N-[4-[[4-[(4-ethoxyphenyl)amino]phenyl][4-[ethyl[(3-sulfophenyl)methyl]amino]phenyl]methylene]-2,5-cyclohexadien-1-ylidene]-N-ethyl-3-sulfo-, inner salt, sodium salt (1:1)
  • RefChem:578669
  • DTXCID801339658
  • 228-060-5
  • Benzenemethanaminium, N(4((4((4ethoxyphenyl)amino)phenyl)(4(ethyl((3sulfophenyl)methyl)amino)phenyl)methylene)2,5cyclohexadien1ylidene)Nethyl3sulfo, hydroxide, inner salt, monosodium salt
  • Benzenemethanaminium, N(4((4((4ethoxyphenyl)amino)phenyl)(4(ethyl((3sulfophenyl)methyl)amino)phenyl)methylene)2,5cyclohexadien1ylidene)Nethyl3sulfo, inner salt, monosodium salt
  • Hydrogen (4(4(pethoxyanilino)4'(ethyl(msulphonatobenzyl)amino)benzhydrylene)cyclohexa2,5dien1ylidene)(ethyl)(msulphonatobenzyl)ammonium, monosodium salt
  • Acid Blue 83
  • Brilliant Blue R250
  • CBBR
  • Coomassie blue R-250
  • Coomassie Brilliant Blue R-250
  • MFCD00041762
  • Benzenemethanaminium,N-[4-[[4-[(4-ethoxyphenyl)amino]phenyl][4-[ethyl[(3-sulfophenyl)methyl]amino]phenyl]methylene]-2,5-cyclohexadien-1-ylidene]-N-ethyl-3-sulfo-,inner salt, monosodium salt
  • CHEMBL4279842
  • Acid Cyanine 6B
  • Solar Cyanine 6B
  • Alizarin Rubinol 5G
  • AcidBlue83
  • Coomassie Blue R-250, Technical Grade
  • N-[4-[[4-[(4-Ethoxyphenyl)amino]phenyl][4-[ethyl[(3-sulfophenyl)methyl]amino]phenyl]methylene]-2,5-cyclohexadien-1-ylidene]-N-ethyl-3-sulfo-benzenemethanaminium inner salt, sodium salt (1:1)
  • sodium;3-[[4-[[4-(4-ethoxyanilino)phenyl]-[4-[ethyl-[(3-sulfonatophenyl)methyl]azaniumylidene]cyclohexa-2,5-dien-1-ylidene]methyl]-N-ethylanilino]methyl]benzenesulfonate
  • C45H44N3NaO7S2
  • Kenacid blue
  • Coomassie Brilliant Blue R250
  • bril-liant blue r
  • Brilliant Blue R-250
  • UNII-TKL1K0PT9A
  • 108066-47-3
  • sodium 3-[[4-[[4-(4-ethoxyanilino)phenyl]-[4-[ethyl-[(3-sulfonatophenyl)methyl]azaniumylidene]cyclohexa-2,5-dien-1-ylidene]methyl]-N-ethylanilino]methyl]benzenesulfonate
  • C45H44N3O7S2Na
  • CBB R250
  • orb2279218
  • SCHEMBL1925726
  • SCHEMBL29354375
  • CBB R-250
  • BDBM50464535
  • HB0739
  • AKOS015902422
  • AKOS037643325
  • FA40938
  • AS-13946
  • DA-61882
  • SY060135
  • B3194
  • D78484
  • F988481
  • Q418516
  • Brilliant blue R staining solution (ethanol solution)
  • Brilliant blue R; Coomassie brilliant blue R-250; Acid Blue 83; CI 42660
  • Sodium 3-({[(1E,4E)-4-{[4-(4-ethoxyanilino)phenyl](4-{ethyl[(3-sulfonatophenyl)methyl]amino}phenyl)methylidene}cyclohexa-2,5-dien-1-ylidene](ethyl)azaniumyl}methyl)benzene-1-sulfonate
  • sodium;3-[[4-[[4-(4-ethoxyanilino)phenyl]-[4-[ethyl-[(3-sulfonatophenyl)methyl]iminio]cyclohexa-2,5-dien-1-ylidene]methyl]-N-ethyl-anilino]methyl]benzenesulfonate

Applications:

Uv-Vis Spectrum of Brilliant Blue R

April 7, 2026

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If you are looking for optimized HPLC method to analyze Brilliant Blue R check our HPLC Applications library

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase.  The compound was dissolved with water. This analysis was done with the mobile phase made of 20% acetonitrile and 80% water.

 

 

Application Analytes:
Brilliant Blue R
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.