Alltesta HPLC Method for Analysis of Linear PEI + (Cu) on PEI Column

Alltesta HPLC Method for Polyethylenimine PEI on PEI by SIELC Technologies

HPLC Method for Analysis of Polyethylenimine PEI on PEI Column by SIELC Technologies

Polyethylenimine (PEI), also known as polyaziridine, is a polymer with the molecular formula (C2H5N)n. It has repeating units of the amine group and carbon aliphatic spacers. PEI has a large variety of uses. Most commonly, it is used in detergents, adhesives, water treatment agents, and cosmetics. Medically, due to being a catatonic polymer, it has been also studied as a gene delivery vehicle with successful outcomes. It is difficult to analyze in HPLC due to several factors. It’s a mixture of compounds with different lengths and therefore different number of charges. It also lacks a UV chromophore.

New PEI column was designed specifically for the analysis of Polyethylenimine PEI by ion-exclusion and size-exclusion mechanisms with copper complex to allow for UV detection. The method uses a mobile phase of acetonitrile (ACN) and water with ammonium formate buffer (AmFm) and UV detection at 275nm

Condition

ColumnPEI, 4.6 x 250 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O – 40/60%,
BufferAmmonium formate pH 3.0 – 20 mM
Flow Rate0.5 ml/min
DetectionUV 275 nm

Description

Class of CompoundsPolymer, Basic, Hydrophilic, Ionizable
Analyzing CompoundsPolyethylenimine PEI

Application Column

PEI

Column Diameter: 4.6 mm
Column Length: 250 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Polyethylenimine PEI
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.