Alltesta HPLC Method for Analysis of Linear PEI + (Cu) on PEI Column

Alltesta HPLC Method for Polyethylenimine PEI on PEI by SIELC Technologies

Separation type: Liquid Chromatography Reversed-phase

HPLC Method for Analysis of Polyethylenimine PEI on PEI Column by SIELC Technologies

Polyethyleneimine (PEI) is a polymer used in a wide range of applications. It is difficult to analyze in HPLC due to several factors. It’s a mixture of compounds with different lengths and therefore different number of charges. It also lacks a UV chromophore. New PEI column was designed specifically for the analysis of polyethyleneimine by ion-exclusion and size-exclusion mechanisms with copper complex to allow for UV detection. The method uses a mobile phase of acetonitrile (ACN) and water with ammonium formate buffer (AmFm) and UV detection at 275nm

High Performance Liquid Chromatography (HPLC) Method for Analyses of Polyethylenimine PEI on PEI Column by SIELC Technologies

Condition

ColumnPEI, 4.6 x 250 mm, 5 µm, 100 A
Mobile PhaseMeCN/H2O – 40/60%,
BufferAmmonium formate pH 3.0 – 20 mM
Flow Rate0.5 ml/min
DetectionUV 275 nm

Description

Class of CompoundsPolymer, Basic, Hydrophilic, Ionizable
Analyzing CompoundsPolyethylenimine PEI

Application Column

PEI

Column Diameter: 4.6 mm
Column Length: 250 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Polyethylenimine PEI
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.