| CAS Number | 79-06-1 |
|---|---|
| Molecular Formula | C3H5NO |
| Molecular Weight | 71.080 |
| InChI Key | HRPVXLWXLXDGHG-UHFFFAOYSA-N |
| LogP | -0.670 |
| Synonyms |
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Applications:
Uv-Vis Spectrum of Acrylamide
January 28, 2026
If you are looking for optimized HPLC method to analyze Acrylamide check our HPLC Applications library
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.
HPLC Separation of Acrylamide and Melamine on Primesep 200 Column
September 4, 2020
HPLC Method for Acrylamide, Melamine on Primesep 200 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Acrylamide, Melamine.
Melamine is a nitrogen-based organic compound that was first synthesized by the German chemist Justus von Liebig in 1834. Melamine is a decorative coating that is resistant to water and mechanical damage.
Melamine is used for:
- lamination of particle boards and plywood;
- manufacture of paints and varnishes;
- production of decorative paper-laminated plastics and plastics;
- manufacture of dishes.
We were able to separate Melamine from Acrylamide in HPLC on Primesep 200 column using a mobile phase of water with Formic Acid buffer. This separation can be monitored with UV, ELSD, CAD detection techniques.
| Column | Primesep 200, 4.6 x 150 mm, 3 µm, 100 A, dual ended |
| Mobile Phase | H2O – 99% |
| Buffer | Formic Acid – 1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 240 nm |
| Class of Compounds | Amide, Amine |
| Analyzing Compounds | Acrylamide, Melamine |
Application Column
Primesep 200
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended
Melamine
Separation of Acrylamide on Newcrom R1 HPLC column
February 16, 2018
Acrylamide is a colorless, white crystalline solid with no odor. It is highly toxic and is used to make polymers, wrinkle-less fabrics, and paper. Acrylamide can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particle columns are available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation of impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select options