HPLC Method for Analysis of Cocaine

Chr_1041 Cocaine, also known as coke, is mostly used as a recreational drug. It is a strong stimulant. The drug’s most common appearance in powder form is a salt, usually cocaine hydrochloride. Due to Cocaine rapidly metabolizing in the body, so if it’s ingested/inhaled, its concentration in urine usually does not exceed 10% that of its principal metabolite, benzoylecgonine. Primesep 200, a reverse phase column, contains embedded acidic ionizable groups and can retain Cocaine. The method is UV compatible and can be used as a general approach for analyzing similar compounds.


Column Primesep 200, 3.2x100 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 5-50%
Buffer Gradient H3PO4 - 0.05-0.3%, 5 min, 4 min hold
Flow Rate 0.5 ml/min
Detection UV, 270 nm


Class of Compounds Drug,  Acid, Hydrophilic, Ionizable,
Analyzing Compounds Cocaine

Application Analytes: