HPLC Separation of Lecithin and Soy lecithin on Primesep N Column

Lecithin is a naturally occurring compound composed of phosphoric acid, choline, fatty acids, glycerol, glycolipids, triglycerides and phospholipids.

Condition
Column Primesep N, 4.6×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN 92-75%, 20 min
Buffer Gradient AmFm pH 3.0- 8-20 mM, 20 min
Flow Rate 1.0 ml/min
Detection ELSD, 40C

 

Description
Class of Compounds
 Aminoacid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Lecithin

 

Application Column

Primesep N

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Lecithin
Soy Lecithin
Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.