HPLC Separation of Lecithin and Soy lecithin on Primesep N Column

Lecithin is a naturally occurring compound composed of phosphoric acid, choline, fatty acids, glycerol, glycolipids, triglycerides and phospholipids.

Condition

Column Primesep N, 4.6x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN 92-75%, 20 min
Buffer Gradient AmFm pH 3.0- 8-20 mM, 20 min
Flow Rate 1.0 ml/min
Detection ELSD, 40C
 

Description

Class of Compounds Aminoacid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Lecithin
 
SIELC Technologies usually develops more than one method for each compound on our website. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound of interest.
Application Analytes:
Lecithin
Soy Lecithin

Application Detection:
ELSD/MS Detection