HPLC Separation of Lecithin and Soy lecithin on Primesep N Column

Lecithin is a naturally occurring compound composed of phosphoric acid, choline, fatty acids, glycerol, glycolipids, triglycerides and phospholipids.


Column Primesep N, 4.6x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN 92-75%, 20 min
Buffer Gradient AmFm pH 3.0- 8-20 mM, 20 min
Flow Rate 1.0 ml/min
Detection ELSD, 40C


Class of Compounds Aminoacid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Lecithin
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Application Analytes:
Soy Lecithin

Application Detection:
ELSD/MS Detection