HPLC Method for Folpet on Primesep B2 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Folpet.
Folpet is a nonsystemic fungicide which is used to prevent apple scab, cherry leaf spot, rose black spot, and rose mildew. A method for folpet was provided by the EURL-SRM, a proficiency test for sigle residue methods developed by the European Union Reference Laboratory. Folpet was retained and separated from impurities with Obelisc R and Primesep B2 columns. Primesep B2 contains embedded basic ion-pairing groups and Obelisc R contains embedded ionic and hydrophobic groups which can assist in fine tuning separations. Method can be used for dozens of other pesticides and is LC/MS compatible.
| Column | Primesep B2, 2.1 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | Gradient MeCN – 10-70%, 15 min, 7 min hold |
| Buffer | Gradient AmAc pH 4,5- 20-50 mM, 15 min, 7 min hold |
| Flow Rate | 0.4 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds |
Insecticide, Herbicide, Fungicide, Hydrophobic, Ionizable |
| Analyzing Compounds | Folpet |
Application Column
Primesep B2
Column Diameter: 2.1 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
