HPLC Retention of Pirimicarb on Primesep 100 and Obelisc R

  Pirimicarb is an insecticide that controls aphids on vegetable, cereal, and orchard crops. A carbamate insecticide, pirimicarb inhibits activity of the hydrolase acetylcholinesterase. Pirimicarb is a target pesticide in the EURL (European Union Reference Laboratory) EUPT-FF9 2015 a proficiency test for the analysis of a over a hundred pesticides.  Primesep 100 and Obelisc R were used to retain pirimicarb and separate it from impurities. Primesep 100 contains embedded acidic ion-pairing groups and Obelisc R contains embedded ionic and hydrophobic groups which can assist in fine tuning separations. Method is LC/MS compatible and can be used as a general approach for analyzing pirimicarb, other organophosphate insecticides, and dozens of other pesticides.

Condition 1

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 25-70%, 15 min
Buffer Gradient AmAc pH 3.0- 30-60 mM, 15 min
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Condition 2

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%, 15 min
Buffer Gradient AmAc pH 3.0- 20-60 mM, 15 min
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Condition 3

Column Primesep 100, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%, 15 min
Buffer Gradient AmAc pH 3.0- 20-60 mM, 15 min
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds  Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds Pirimicarb
 
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Application Analytes:
Pirimicarb

Application Detection:
UV Detection