2-Amino-4-nitrophenol

2-Amino-4-nitrophenol structural formula

CAS Number99-57-0
Molecular FormulaC6H6N2O3
Molecular Weight154.125
InChI KeyVLZVIIYRNMWPSN-UHFFFAOYSA-N
LogP1.26
Synonyms
  • 2-Amino-4-nitrophenol
  • Phenol, 2-amino-4-nitro-
  • 99-57-0
  • Phenol, 2-amino-4-nitro-
  • 1-Amino-2-hydroxy-5-nitrobenzene
  • 1-Hydroxy-2-amino-4-nitrobenzene
  • 2-amino-4-nitrofenol
  • 2-Hydroxy-5-nitroaniline
  • 4-Nitro-2-amino-1-hydroxybenzene
  • 4-Nitro-2-aminophenol
  • 5-Nitro-2-hydroxyaniline
  • NSC 4664
  • p-Nitro-o-aminophenol
  • 3-Amino-4-hydroxynitrobenzene
  • BRN 0776533
  • EINECS 202-767-9
  • NCI-C55958
  • 1-Nitro-3-amino-4-hydroxybenzene
  • 4-Nitro-2-aminofenol
  • p-Nitroaminofenol
  • UNII-G501UCI6T9

Applications:

UV-Vis Spectrum of 2-Amino-4-nitrophenol

July 11, 2025
UV-Vis Spectrum of 2-Amino-4-nitrophenol. Absorption Maxima: 224 nm, 262 nm, 308 nm.

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.

Application Analytes:
2-Amino-4-nitrophenol
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of 2-Amino-4-nitrophenol on Newcrom R1 HPLC column

February 16, 2018
Separation of 2-Amino-4-nitrophenol on Newcrom C18 HPLC column

2-Amino-4-nitrophenol can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
2-Amino-4-nitrophenol
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us before ordering the column as there may be a more suitable column alternative by either e-mail: support@sielc.com or by phone: 847-229-2629.