| Molecular Formula | C5H14N2 |
|---|---|
| Molecular Weight | 102.18 |
| InChI Key | VHRGRCVQAFMJIZ-UHFFFAOYSA-N |
| LogP | -0.6 |
| Synonyms |
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Applications:
Uv-Vis Spectrum of 1,5-diaminopentane
February 9, 2026
If you are looking for optimized HPLC method to analyze 1,5-Diaminopentane (Cadaverine) check our HPLC Applications library
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.
HPLC Method Separation of 1,3-Diaminopropane, 1,4-Diaminobutane (Putrescine) and 1,5-Diaminopentane (Cadaverine) on Primesep 200 Column
March 24, 2023
HPLC Method for Analysis of 1,5-Diaminopentane (Cadaverine), 1,3-Diaminopropane, 1,4-Diaminobutane (Putrescine) on Primesep 200 by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode
1,3-Diaminopropane, or trimethylenediamine, is a key heterocycle building block and precursor of the chemotherapy antineoplastic agents Prioxantrone and Losoxantrone. 1,4-Diaminobutane, or Putrescine, is one of the main sources of the foul odor of rotting flesh, but has found use as a building block of Nylon 46. 1,5-Diaminopentane, or Cadaverine, is another source of the foul odor of rotting flesh and since its produced by the decarboxylation of lysine, can be used as a biomarker for lysine metabolic deficiencies.
These (putrid) diamines can be retained, analyzed, and separated on a Primesep 200 column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and an Ammonium formate (AmFm) ionic modifier. This analysis method can be detected with an Evaporative Light Scattering Detector (ELSD) or any other evaporative detection method (CAD, ESI-MS).
High Performance Liquid Chromatography (HPLC) Method for Analysis of 1,5-Diaminopentane (Cadaverine), 1,3-Diaminopropane, 1,4-Diaminobutane (Putrescine)
Condition
| Column | Primesep 200, 4.6 x 100 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | H2O |
| Buffer | Ammonium formate pH 3.0 – 40 mM |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD, 50C |
| Peak Retention Time | 5.2, 5.8, 7.2 min |
Description
| Class of Compounds | Diamino |
| Analyzing Compounds | 1,5-Diaminopentane (Cadaverine), 1,3-Diaminopropane, 1,4-Diaminobutane (Putrescine) |
Application Column
Primesep 200
Column Diameter: 4.6 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
1,4-Diaminobutane (Putrescine)
1,5-Diaminopentane (Cadaverine)
