1,4-Diaminobutane (Putrescine)

1,4-Diaminobutane

CAS Number110-60-1
Molecular FormulaC4H12N2
Molecular Weight88.15
InChI KeyKIDHWZJUCRJVML-UHFFFAOYSA-N
LogP0.1
Synonyms
  • 1,4 Butanediamine
  • 1,4 Diaminobutane
  • 1,4-Butanediamine
  • 1,4-Diaminobutane
  • Putrescine
  • Tetramethylenediamine

Applications:

HPLC ELSD Method for Analysis Putrescine on Obelisc R Column

February 1, 2024

HPLC Method for Analysis of 1,4-Diaminobutane (Putrescine) on Obelisc R by SIELC Technologies

HPLC Method for Analysis of 1,4-Diaminobutane (Putrescine) on Obelisc R Column by SIELC Technologies


 High Performance Liquid Chromatography (HPLC) Method for Analysis of 1,4-Diaminobutane (Putrescine)

Putrescine is a biogenic amine and organic compound with the chemical formula. It is a polyamine, which means it contains multiple amino groups. Putrescine is one of the naturally occurring polyamines found in living organisms and is involved in various biological processes.

Biological Roles:

  • Putrescine is involved in cell growth, proliferation, and differentiation.
  • It plays a role in the synthesis of other important molecules, including certain alkaloids and polyamine derivatives.
  • Putrescine is produced by the decarboxylation of ornithine, an amino acid.

Polyamine Family:

  • Putrescine is part of a family of polyamines, which also includes spermidine and spermine.
  • Polyamines are essential for cell growth, and their levels are tightly regulated in living cells.

Research and Applications:

  • Putrescine and other polyamines have been studied for their roles in cancer, neurodegenerative diseases, and other health-related conditions.
  • In some industries, putrescine is used as a chemical intermediate for the production of certain polymers and plastics.

Putrescine can be retained and analyzed using an Obelisc R mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and ammonium formate as a buffer. Detection is achieved using ELSD

ColumnObelisc R, 2.1 x 100 mm, 5 µm, 100 A
Mobile PhaseMeCN/H2O – 50/50%
BufferAmmonium formate pH 3.0 – 20 mM
Flow Rate0.2 ml/min
DetectionELSD, the nebulizer and evaporator temperatures 50°C,
 with a gas flow rate of 1.6 Standard Liters per Minute (SLM)
Samples0.1 mg/ml
Injection volume5 µl
LOD*200 ppb
* LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of Compounds
Polyamines
Analyzing Compounds1,4-Diaminobutane (Putrescine)

Application Column

Obelisc R

Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
1,4-Diaminobutane (Putrescine)
Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method Separation of 1,3-Diaminopropane, 1,4-Diaminobutane (Putrescine) and 1,5-Diaminopentane (Cadaverine) on Primesep 200 Column

March 24, 2023

HPLC Method for Analysis of 1,5-Diaminopentane (Cadaverine), 1,3-Diaminopropane, 1,4-Diaminobutane (Putrescine) on Primesep 200 by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode

HPLC Method Separation of 1,3-Diaminopropane, 1,4-Diaminobutane (Putrescine) and 1,5-Diaminopentane (Cadaverine) on Primesep 200  Column
HPLC Method for Analysis of 1,5-Diaminopentane (Cadaverine), 1,3-Diaminopropane, 1,4-Diaminobutane (Putrescine) on Primesep 200 by SIELC Technologies

1,3-Diaminopropane, or trimethylenediamine, is a key heterocycle building block and precursor of the chemotherapy antineoplastic agents Prioxantrone and Losoxantrone. 1,4-Diaminobutane, or Putrescine, is one of the main sources of the foul odor of rotting flesh, but has found use as a building block of Nylon 46. 1,5-Diaminopentane, or Cadaverine, is another source of the foul odor of rotting flesh and since its produced by the decarboxylation of lysine, can be used as a biomarker for lysine metabolic deficiencies.

These (putrid) diamines can be retained, analyzed, and separated on a Primesep 200 column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and an Ammonium formate (AmFm) ionic modifier. This analysis method can be detected with an Evaporative Light Scattering Detector (ELSD) or any other evaporative detection method (CAD, ESI-MS).

High Performance Liquid Chromatography (HPLC) Method for Analysis of  1,5-Diaminopentane (Cadaverine), 1,3-Diaminopropane, 1,4-Diaminobutane (Putrescine)

Condition

ColumnPrimesep 200, 4.6 x 100 mm, 5 µm, 100 A
Mobile PhaseH2O
BufferAmmonium formate pH 3.0 – 40 mM
Flow Rate1.0 ml/min
DetectionELSD, 50C
Peak Retention Time5.2, 5.8, 7.2 min

Description

Class of CompoundsDiamino
Analyzing Compounds1,5-Diaminopentane (Cadaverine), 1,3-Diaminopropane, 1,4-Diaminobutane (Putrescine)

Application Column

Primesep 200

Column Diameter: 4.6 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A

Add to cart
Application Analytes:
1,3-Diaminopropane
1,4-Diaminobutane (Putrescine)
1,5-Diaminopentane (Cadaverine)
Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.