HPLC Method Separation of 1,3-Diaminopropane, 1,4-Diaminobutane (Putrescine) and 1,5-Diaminopentane (Cadaverine) on Primesep 200 Column

HPLC Method for Analysis of 1,5-Diaminopentane (Cadaverine), 1,3-Diaminopropane, 1,4-Diaminobutane (Putrescine) on Primesep 200 by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode

HPLC Method Separation of 1,3-Diaminopropane, 1,4-Diaminobutane (Putrescine) and 1,5-Diaminopentane (Cadaverine) on Primesep 200  Column
HPLC Method for Analysis of 1,5-Diaminopentane (Cadaverine), 1,3-Diaminopropane, 1,4-Diaminobutane (Putrescine) on Primesep 200 by SIELC Technologies

1,3-Diaminopropane, or trimethylenediamine, is a key heterocycle building block and precursor of the chemotherapy antineoplastic agents Prioxantrone and Losoxantrone. 1,4-Diaminobutane, or Putrescine, is one of the main sources of the foul odor of rotting flesh, but has found use as a building block of Nylon 46. 1,5-Diaminopentane, or Cadaverine, is another source of the foul odor of rotting flesh and since its produced by the decarboxylation of lysine, can be used as a biomarker for lysine metabolic deficiencies.

These (putrid) diamines can be retained, analyzed, and separated on a Primesep 200 column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and an Ammonium formate (AmFm) ionic modifier. This analysis method can be detected with an Evaporative Light Scattering Detector (ELSD) or any other evaporative detection method (CAD, ESI-MS).

High Performance Liquid Chromatography (HPLC) Method for Analysis of  1,5-Diaminopentane (Cadaverine), 1,3-Diaminopropane, 1,4-Diaminobutane (Putrescine)

Condition

ColumnPrimesep 200, 4.6 x 100 mm, 5 µm, 100 A
Mobile PhaseH2O
BufferAmmonium formate pH 3.0 – 40 mM
Flow Rate1.0 ml/min
DetectionELSD, 50C
Peak Retention Time5.2, 5.8, 7.2 min

Description

Class of CompoundsDiamino
Analyzing Compounds1,5-Diaminopentane (Cadaverine), 1,3-Diaminopropane, 1,4-Diaminobutane (Putrescine)

Application Column

Primesep 200

Column Diameter: 4.6 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A

Add to cart
Application Analytes:
1,3-Diaminopropane
1,4-Diaminobutane (Putrescine)
1,5-Diaminopentane (Cadaverine)

Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.