HPLC Method for Analysis of 1,4-Diaminobutane (Putrescine) on Obelisc R by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of 1,4-Diaminobutane (Putrescine)
Putrescine is a biogenic amine and organic compound with the chemical formula. It is a polyamine, which means it contains multiple amino groups. Putrescine is one of the naturally occurring polyamines found in living organisms and is involved in various biological processes.
Biological Roles:
- Putrescine is involved in cell growth, proliferation, and differentiation.
- It plays a role in the synthesis of other important molecules, including certain alkaloids and polyamine derivatives.
- Putrescine is produced by the decarboxylation of ornithine, an amino acid.
Polyamine Family:
- Putrescine is part of a family of polyamines, which also includes spermidine and spermine.
- Polyamines are essential for cell growth, and their levels are tightly regulated in living cells.
Research and Applications:
- Putrescine and other polyamines have been studied for their roles in cancer, neurodegenerative diseases, and other health-related conditions.
- In some industries, putrescine is used as a chemical intermediate for the production of certain polymers and plastics.
Putrescine can be retained and analyzed using an Obelisc R mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and ammonium formate as a buffer. Detection is achieved using ELSD
| Column | Obelisc R, 2.1 x 100 mm, 5 µm, 100 A |
| Mobile Phase | MeCN/H2O – 50/50% |
| Buffer | Ammonium formate pH 3.0 – 20 mM |
| Flow Rate | 0.2 ml/min |
| Detection | ELSD, the nebulizer and evaporator temperatures 50°C, with a gas flow rate of 1.6 Standard Liters per Minute (SLM) |
| Samples | 0.1 mg/ml |
| Injection volume | 5 µl |
| LOD* | 200 ppb |
| Class of Compounds | Polyamines |
| Analyzing Compounds | 1,4-Diaminobutane (Putrescine) |
Application Column
Obelisc R
Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Application Analytes:
1,4-Diaminobutane (Putrescine)Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
