| CAS Number | 69-72-7 |
|---|---|
| Molecular Formula | C7H6O3 |
| Molecular Weight | 138.122 |
| InChI Key | YGSDEFSMJLZEOE-UHFFFAOYSA-N |
| LogP | 2.26 |
| Synonyms |
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Applications:
HPLC Method For Analysis Of Salicylic Acid on Newcrom BH Column
May 2, 2022
Separation type: Liquid Chromatography Mixed-mode
Salicylic acid (SA) is an o-hydroxybenzoic acid, synthesized by both plants and microorganisms. SA acts as a critical plant hormone regulating various processes, including growth and development, flowering, thermogenesis, ion uptake, stomatal movement, photosynthesis, and plant immunity. SA also has medical applications as a topical treatment for a wide variety of skin conditions, including warts, dandruff, and acne. Using a Newcrom BH mixed-mode column and a mobile phase consisting of water, Acetonitrile (MeCN), and a Phosphoric acid (H3PO4) buffer, Salicylic acid can be retained, measured, and analyzed. This analysis method can be UV detected at 200 nm with high resolution with high resolution and peak symmetry.
High Performance Liquid Chromatography (HPLC) Method for Analysis of Salicylyc Acid
| Column | Newcrom BH, 4.6×150 mm, 100A |
| Mobile Phase | MeCN – 40% |
| Buffer | H3PO4 – 0.5% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200nm |
| Class of Compounds | Acid, Hydrophilic |
| Analyzing Compounds | Salicylic Acid |
Application Column
Newcrom BH
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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HPLC Separation of Cinnamic Acid, Salicylic Acid and Quercetin on Newcrom B Column
March 12, 2021
Separation type: Liquid Chromatography Mixed-mode
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High Performance Liquid Chromatography (HPLC) Method of Cinnamic Acid, Salicylic Acid and Quercetin.
Cinnamic acid and benzoic acid are two of the major phenolic acids and autotoxins. Cinnamic acid occurs naturally in all green plants, showing significant antimicrobial activity against both bacteria and fungi as well as antioxidant activity. Salicylic acid (SA) is an o-hydroxybenzoic acid, synthesized by both plants and microorganisms. SA acts as a critical plant hormone regulating various processes, including growth and development, flowering, thermogenesis, ion uptake, stomatal movement, photosynthesis, and plant immunity. Quercetin is a naturally occurring flavonol, or flavonoid, the yellowish antioxidant pigment found in skins of red grapes, berries, apples, onions, tomatoes, and buckwheat tea. In addition to functioning as a flavonoid, quercetin is also a phytoestrogen. All three compounds can be retained and separated by using a mixed-mode Newcrom B column using an isocratic analytical method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and phosphoric acid (H3PO4) buffer. UV detection at 250 nm.
| Column | Newcrom B, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN -50% |
| Buffer | H3PO4 |
| Flow Rate | 1.0 ml/min |
| Detection | 250 nm |
| Class of Compounds | Acid |
| Analyzing Compounds | Cinnamic acid, Quercetin, Salicylic acid |
Application Column
Newcrom B
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsQuercetin
Quercetin dihydrate
Salicylic acid
HPLC Separation of Phenolic Acids
March 11, 2021
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| Column | Newcrom B, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | Gradient MeCN |
| Buffer | H3PO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | 270 nm |
| Column | Newcrom R1, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | Gradient MeCN |
| Buffer | H3PO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | 270 nm |
| Class of Compounds | Acid |
| Analyzing Compounds | Gallic acid, 3,4 Dihydroxybenzoic acid, Caffeic acid, trans-Ferulic acid, trans- p -Coumaric acid, trans-Cinnamic acid, Quercetin Hydrate, Salicylic acid |
Application Column
Newcrom B
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsCaffeic acid
Cinnamic acid
Ferulic acid
Gallic acid
Quercetin
Quercetin dihydrate
Salicylic acid
p-coumaric acid
HPLC Separation of Mixture of 2-Hydroxyethyl Salicylate and Salicylic Acid on Primesep B Column
August 14, 2019
2-Hydroxyethyl salicylate is a drug used as analgesic agents for the treatment of mild to moderate pain.
| Column | Primesep B, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 25/75% |
| Buffer | H2SO4 – 0.5 % |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 238 nm |
| Class of Compounds |
Acid, Hydrophilic, Ionizable, Carboxylic acid, Drug, Carbocyclic, Benzoate, Hydroxy Acid, Hydroxybenzoate, Phenol |
| Analyzing Compounds | 2-Hydroxyethyl Salicylate, Salicylic Acid |
Application Column
Primesep B
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsSalicylic acid
HPLC Separation of Salicylic acid
August 6, 2015
Salicylic acid is a pain-reliever and anti-inflammatory commonly found in rubefacient products for topical pain relief as well as cosmetic skin-care products used to treat a variety of skin conditions. Legacy L1 was used to retain salicylic acid by reverse phase mechanism.Legacy L1 uses embedded C18 groups on porous silica and is useful for many USP HPLC applications. comparisons to Phenomenex columns are available by request.
| Column | Legacy L1, 4.6×250 mm, 5 µm, 100A |
| Mobile Phase | MeOH – 45% |
| Buffer | Acetic acid – 0.55% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 280 nm |
| Class of Compounds |
Acid, Hydrophilic, Ionizable |
| Analyzing Compounds | Salicylic acid |
Application Column
Legacy L1
SIELC's family of Legacy columns is based on the United States Pharmacopeia's (USP) published chromatographic methods and procedures. Numerous brands have columns used in USP reference standards and methods. USP has created various designations to group together columns with similar types of packing and properties in the solid phase. SIELC's Legacy columns adhere to these strict requirements and properties, allowing you to easily replace older columns that are no longer available without needing to significantly modify your method or SOPs.
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