HPLC Method For Analysis Of Salicylic Acid on Newcrom BH Column

HPLC Method for Salicylic acid on Newcrom BH by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Salicylic acid.

Salicylic acid (SA) is an o-hydroxybenzoic acid, synthesized by both plants and microorganisms. SA acts as a critical plant hormone regulating various processes, including growth and development, flowering, thermogenesis, ion uptake, stomatal movement, photosynthesis, and plant immunity. Medically, Salicylic acid is used as a topical treatment for a wide variety of skin conditions. It has the chemical formula C7H6O3. You can find detailed UV spectra of Salicylic acid and information about its various lambda maxima by visiting the following link.

Salicylic acid can be retained and analyzed using the Newcrom BH stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a phosphoric acid buffer. Detection is performed using UV.

Condition
Column Newcrom BH, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN – 40%
Buffer H3PO4 – 0.5%
Flow Rate 1.0 ml/min
Detection UV 200nm

Description
Class of Compounds Acid, Hydrophilic
Analyzing Compounds Salicylic acid

 

Application Column

Newcrom BH

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Salicylic acid
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.