HPLC Method for Analysis of Warfarin on Cyralis CM Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Warfarin.

Warfarin is an anticoagulant, also known as blood thinners, medication with the chemical formula C₁₀H₁₄O₄. It is used to prevent deep vein thrombosis and stoke through inhibitying the vitamin K-dependent synthesis of clotting factors II, VII, IX, and X. You can find detailed UV spectra of Warfarin and information about its various lambda maxima by visiting the following link.

This application describes a normal-phase chiral HPLC method for the separation of Warfarin enantiomers using SIELC’s Cyralis CM chiral column. Cyralis CM is a cellulose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications. When preparing a sample for analysis, it is crucial for the sample to be made within the same day.

Warfarin can be retained and analyzed using the Cyralis CM stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of Ethanol and AcOH. Detection is performed using UV.

Condition

Column	Cyralis CM, 4.6 x 100 mm, 5 µm, 100 A, dual ended
Mobile Phase	EtOH/AcOH- 100/0.01%
Flow Rate	1.0 mL/min
Detection	UV 250 nm
Resolution	2.70

Description

Class of Compounds	Anticoagulant
Analyzing Compounds	Warfarin

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If you are looking for optimized HPLC method to analyze Warfarin check our HPLC Applications library

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.

 

HPLC Method for Warfarin, Sodium warfarin on Newcrom R1 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Warfarin, Sodium warfarin Warfarin is a medication that is also known as “blood thinner”. It is used to treat and prevent blood clots. Blood clots can heighten the risk of a stroke or a heart attack which could be fatal. Newcrom R1, a column that takes advantage of the newest technologies, does not contain embedded acidic nor basic ionizable groups and can retain Warfarin. The method is UV compatible and can be used as a general approach for analyzing similar compounds.

Column	Newcrom R1, 3.2 x 150 mm, 3 µm, 100 A, dual ended
Mobile Phase	MeCN – 50%
Buffer	Phosphoric Acid
Flow Rate	0.5 ml/min
Detection	UV 270 nm

Class of Compounds	Medication
Analyzing Compounds	Warfarin, Sodium warfarin