HPLC Chiral Separation of Warfarin on Cyralis CM Column

HPLC Method for Analysis of Warfarin on Cyralis CM Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Warfarin.

Warfarin is an anticoagulant, also known as blood thinners, medication with the chemical formula C10H14O4. It is used to prevent deep vein thrombosis and stoke through inhibitying the vitamin K-dependent synthesis of clotting factors II, VII, IX, and X. You can find detailed UV spectra of Warfarin and information about its various lambda maxima by visiting the following link.

This application describes a normal-phase chiral HPLC method for the separation of Warfarin enantiomers using SIELC’s Cyralis CM chiral column. Cyralis CM is a cellulose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications. When preparing a sample for analysis, it is crucial for the sample to be made within the same day.

Warfarin can be retained and analyzed using the Cyralis CM stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of Ethanol and AcOH. Detection is performed using UV.

Condition

ColumnCyralis CM, 4.6 x 100 mm, 5 µm, 100 A, dual ended
Mobile PhaseEtOH/AcOH- 100/0.01%
Flow Rate1.0 mL/min
DetectionUV 250 nm
Resolution2.70

Description

Class of CompoundsAnticoagulant
Analyzing CompoundsWarfarin

Application Column

Cyralis CM

Column Diameter: 4.6 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Warfarin
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.