Phosphonoacetic acid

Phosphonoacetic acid structural formula

CAS Number4408-78-0
Molecular FormulaC2H5O5P
Molecular Weight140.032
InChI KeyXUYJLQHKOGNDPB-UHFFFAOYSA-N
LogP-1.59
Synonyms
  • Phosphonoacetic acid
  • Acetic acid, 2-phosphono-
  • 4408-78-0
  • 4-04-00-03612
  • BRN 1764355
  • Carboxymethanephosphonic acid
  • EINECS 224-558-1
  • NSC 18205
  • Phosphonacetic acid
  • UNII-N919E46723
  • 2-phosphonoacetic acid
  • 2-phosphonoethanoic acid
  • Fosfonet

Applications:

HPLC MS Method for Analysis of Phosphonoacetic Acid on Chromni Column

August 12, 2026

HPLC Method for Phosphonoacetic acid on Chromni by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Phosphonoacetic acid

Phosphonoacetic Acid, also written as PAA, is an organophosphorus compound with the chemical formula C2H5O5P. It is used primarily in research as a targeted inhibitor of DNA polymerase in herpesviruses, cytomegalovirus, and orthopoxyviruses. It is also a structural precursor and model in broad-spectrum antiviral agents.

Phosphonoacetic acid can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) with an sulfuric acid as a buffer. Detection is performed using UV.

ColumnChromni, 4.6 x 150 mm, 3 µm, 100 A, surface coated
Mobile PhaseMeCN/H2O – 75/25%
BufferH2SO4 – 0.1%
Flow Rate1.0 ml/min
DetectionUV 210 nm
Class of Compounds
 Acid
Analyzing CompoundsPhosphonoacetic acid

Application Column

Chromni

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: surface coated

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Application Analytes:
Phosphonoacetic acid
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Phosphonoacetic acid on Newcrom R1 HPLC column

February 16, 2018
Separation of Phosphonoacetic acid on Newcrom C18 HPLC column

Phosphonoacetic acid can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Phosphonoacetic acid
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us before ordering the column as there may be a more suitable column alternative by either e-mail: support@sielc.com or by phone: 847-229-2629.