Lithocholic acid

Lithocholic acid structural formula

CAS Number434-13-9
Molecular FormulaC24H40O3
Molecular Weight376.581
InChI KeySMEROWZSTRWXGI-HVATVPOCSA-N
LogP5.45
Synonyms
  • Lithocholic acid
  • (3alpha,5beta)-3-Hydroxycholan-24-oic acid
  • Cholan-24-oic acid, 3-hydroxy-, (3alpha,5beta)-
  • 434-13-9
  • 4-10-00-00785
  • Cholan-24-oic acid, 3-hydroxy-, (3?,5?)-
  • 17?-(1-Methyl-3-carboxypropyl)etiocholan-3?-ol
  • 3-Hydroxycholan-24-oic acid
  • 3?-Hydroxy-5?-cholan-24-oate
  • 3?-Hydroxy-5?-cholan-24-oic acid
  • 3?-Hydroxy-5?-cholanic acid
  • 3?-Hydroxy-5?-cholanoic acid
  • 3?-Hydroxycholanic acid
  • 5?-Cholan-24-oic acid, 3?-hydroxy-
  • 5?-Cholanic acid, 3?-hydroxy-
  • Acide lithocholique
  • acido litocolico
  • Lithocholsaure
  • NSC 683770
  • BRN 3217757
  • 5-beta-Cholanic acid, 3-alpha-hydroxy-
  • 5beta-Cholan-24-oic acid, 3alpha-hydroxy-
  • EINECS 207-099-1
  • 3-alpha-Hydroxycholanic acid
  • 3alpha-Hydroxycholanic acid
  • 3-alpha-Hydroxy-5-beta-cholanic acid
  • 3alpha-Hydroxy-5beta-cholan-24-oic acid
  • (3-alpha,5-beta)-3-Hydroxycholan-24-oic acid
  • 17-beta-(1-Methyl-3-carboxypropyl)ethiocholan-3-alpha-ol
  • 17beta-(1-Methyl-3-carboxypropyl)etiocholan-3alpha-ol
  • NCI-C03861
  • NSC 657956
  • UNII-5QU0I8393U
  • (3a,5b)-3-hydroxy-cholan-24-oate
  • (3a,5b)-3-hydroxy-cholan-24-oic acid
  • (3alpha,5beta)-3-hydroxycholan-24-oic acid
  • (4R)-4-[(1S,2S,5R,10R,11S,14R,15R)-5-hydroxy-2,15-dimethyltetracyclo[8.7.0.02,7.011,15]heptadecan-14-yl]pentanoic acid
  • (4R)-4-[(3R,5R,8R,9S,10S,13R,14S,17R)-3-hydroxy-10,13-dimethyl-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-17-yl]pentanoic acid
  • (4R)-4-[(3R,5R,8R,9S,10S,13R,14S,17R)-3-hydroxy-10,13-dimethyl-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-17-yl]valeric acid
  • 3a-Hydroxy-5b-cholan-24-oate
  • 3a-Hydroxy-5b-cholan-24-oic acid
  • 3alpha-Hydroxy-5beta-cholanate
  • 3alpha-hydroxy-5beta-cholanic acid
  • 3alpha-hydroxy-5beta-cholanoic acid
  • 5beta-cholanic acid-3alpha-ol

Applications:

Separation of Lithocholic acid on Newcrom R1 HPLC column

February 16, 2018
Separation of Lithocholic acid on Newcrom C18 HPLC column

Lithocholic acid can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Lithocholic acid
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: support@sielc.com or by phone: 847-229-2629.