Isoflavone

Isoflavone structural formula

CAS Number574-12-9
Molecular FormulaC15H10O2
Molecular Weight222.243
InChI KeyGOMNOOKGLZYEJT-UHFFFAOYSA-N
LogP3.34
Synonyms
  • Isoflavone
  • 3-Phenyl-4H-1-benzopyran-4-one
  • 4H-1-Benzopyran-4-one, 3-phenyl-
  • 574-12-9
  • NSC 135405
  • UNII-OVO2KUW8H8
  • 3-phenyl-4H-1-benzopyran-4-one
  • Isoflavon

Applications:

Separation of Isoflavone on Newcrom R1 HPLC column

May 17, 2018
Separation of Isoflavone on Newcrom R1 HPLC column

Isoflavone is the parent molecule of a branch of compounds called isoflavones. Isoflavones are touted to have many health benefits. Isoflavone can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particle columns are available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation of impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Isoflavone
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us before ordering the column as there may be a more suitable column alternative by either e-mail: support@sielc.com or by phone: 847-229-2629.