HPLC Method for Pyrazine, 2-Aminopyrazine on SHARC 1 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Pyrazine, 2-Aminopyrazine
Pyrazine is an organic compound with C₄H₄N₂ chemical formula. It is known for its sweet and nutty aroma, but is usually used as a therapeutic agent in several drugs. Derivatives of it are also said to have anti-inflammatory, anticancer, antibacterial, and antioxidant properties.
Aminopyrazine, also known as 2-aminopyrazine and pyrazinamide, is a Pyrazine derivative with C₄H₅N₃ chemical formula. It is typically used as a building block in chemical synthesis and is found in pharmaceuticals, especially in antiviral drugs and other agrochemicals. Structure wise, it is a pyrazine ring with an attached amine group.
Pyrazine, 2-Aminopyrazine can be retained and analyzed using the SHARC 1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a formic acid buffer. Detection is performed using UV.
| Column | SHARC 1, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 98/2% |
| Buffer | Formic Acid – 0.5 % AmFm -0.05% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 270 nm |
| Class of Compounds | Hydrophilic, Heterocyclic, Aromatic |
| Analyzing Compounds | Pyrazine, 2-Aminopyrazine |
Application Column
SHARC 1
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Pyrazine
