HPLC Separation Of Mixture Of Pyridylacetic Acids (2-Pyridylacetic acid, 3-Pyridylacetic acid, 4-Pyridylacetic acid) On Primesep C Column

HPLC Separation Of Mixture of Pyridylacetic Acids

 

Condition
Column Primesep C, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 10/90%
Buffer AmFm pH 4.0- 10 mM
Flow Rate 1.0 ml/min
Detection UV, 270 nm

 

Description
Class of Compounds
 Acid, Hydrophilic, Ionizable,  Metabolite of nicotine and other tobacco alkaloids
Analyzing Compounds 2-Pyridylacetic acid hydrochloride
3-Pyridylacetic acid hydrochloride
4-Pyridylacetic acid hydrochloride

 

Application Column

Primesep C

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
2-Pyridylacetic acid hydrochloride
3-Pyridylacetic acid hydrochloride
4-Pyridylacetic acid hydrochloride
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.