HPLC Separation of Cytidine-5′-diphosphate (CDP) and Adenosine 5′-Triphosphate (ATP) on Newcrom B Column

HPLC Method for Adenosine Triphosphate, Cytidine Diphosphate on Newcrom B by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Adenosine Triphosphate, Cytidine Diphosphate .

Cytidine-5′-diphosphate is a nucleoside diphosphate with C9H15N3O11P2 chemical formula. It is used by RNA and DNA to draw upon it for building blocks. It is a precursor to cytidine triphosphate.

Adenosine 5′-Triphosphate is a nucleotide with the chemical formula C10H16N5O13P3. It is referred to as “energy currency” of the cell due to it’s importance in cellular energy metabolism. It is generated in the mitochondria and is used in numerous various cellular processes.

Adenosine Triphosphate, Cytidine Diphosphate can be retained and analyzed using the Newcrom B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a formic acid buffer. Detection is performed using UV.

Condition
Column Newcrom B, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN/H2O – 10/85%
Buffer Formic Acid –  5%
Flow Rate 1.0 ml/min
Detection UV 270nm

Description
Class of Compounds Acid, Hydrophilic, Ionizable
Analyzing Compounds Adenosine Triphosphate, Cytidine Diphosphate

 

Application Column

Newcrom B

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Adenosine Triphosphate
Cytidine Diphosphate
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.