HPLC Method for Analysis of Biopterin on Primesep 100 Column

HPLC Method for Analysis of Biopterin on Primesep 100 by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode

HPLC Method for Analysis of Biopterin on Primesep 100 Column  by SIELC Technologies


Biopterin belongs to the chemical group known as pteridines. Pteridines are aromatic compounds that consist of a pyrimidine ring fused to a pyrazine ring. These compounds are involved in various biological processes and can be found in different organisms, including bacteria, plants, and animals.

Biopterin, specifically, plays a critical role in the body as a cofactor in the synthesis of several important neurotransmitters, including serotonin, melatonin, dopamine, norepinephrine, and epinephrine. These neurotransmitters are crucial for many physiological functions, including mood regulation, sleep, and response to stress.

Deficiency in biopterin metabolism can lead to various disorders, such as neurodegeneration due to BH4 deficiency, a group of rare inherited neuro-metabolic disorders. This is why understanding and potentially modulating biopterin and its derivatives could be significant for certain therapeutic approaches.

Biopterin retained and analyzed using a reverse-phase Primesep 100, 4.6 x 150 mm, 5 µm, 100 A column. The mobile phase for this method consists of water, acetonitrile (MeCN), and ammonium formate, which serves as a buffer. This analytical method can be monitored using UV detection at 290 nm, an Evaporative Light Scattering Detector (ELSD), or any other evaporative detection method such as Charged Aerosol Detection (CAD) or Electrospray Ionization Mass Spectrometry (ESI-MS)

LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

High Performance Liquid Chromatography (HPLC) Method for Analysis of Biopterin

Condition

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN/H2O -5/95%
BufferAmmonium formate pH 3.0 – 20 mM
Flow Rate1.0 ml/min
DetectionUV 275 nm
Peak Retention Time4.78 min
Sample concentration0.5 mg/ml
Injection volume1 µl
Sample diluent H2O + NaOH
LOD20 ppb

Description

Class of Compounds Pteridines
Analyzing CompoundsBiopterin

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Biopterin

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.