HPLC Method for Separation of S-Nitroso-L-glutathione and Glutathione oxidized on Primesep 100  Column

HPLC Method for Separation of S-Nitroso-L-glutathione and Glutathione oxidized on Primesep 100 by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode

HPLC Method for Separation of S-Nitroso-L-glutathione and Glutathione oxidized on Primesep 100  Column by SIELC Technologies
HPLC Method for Separation of S-Nitroso-L-glutathione and Glutathione oxidized on Primesep 100  Column

Both S-Nitroso-L-glutathione (GSNO) and oxidized glutathione (GSSG) are forms of glutathione, a tripeptide consisting of the amino acids glutamic acid, cysteine, and glycine. Glutathione plays a significant role in maintaining the redox (reduction-oxidation) balance in cells, but GSNO and GSSG each have unique characteristics:

  1. S-Nitroso-L-glutathione (GSNO): GSNO is a nitric oxide (NO) donor in cells, and it is part of the group of S-nitrosothiols. The nitric oxide group is attached to the sulfur atom of the cysteine residue in glutathione. GSNO plays a key role in nitric oxide-mediated cellular signaling and can regulate protein function through a process called S-nitrosylation.
  2. Glutathione, oxidized (GSSG): GSSG is formed when two molecules of glutathione (GSH, the reduced form of glutathione) are linked together by a disulfide bond (-S-S-). This typically occurs in cells under conditions of oxidative stress, when reactive oxygen species (ROS) levels are high. The enzyme glutathione reductase can convert GSSG back to GSH using NADPH as a cofactor, which is an important part of cellular defenses against oxidative stress.

Each form of glutathione plays a unique role in cell signaling and defense mechanisms. While they are related, the specific effects of GSNO and GSSG within cells can be quite different due to their distinct chemical structures and reactivities.

These compounds can be retained, separated, and analyzed using a reverse-phase Primesep 100, 4.6 x 150 mm, 5 µm, 100 A column. The mobile phase for this method consists of water, acetonitrile (MeCN), and Sulfuric acid, which serves as a buffer. This analytical method can be

High Performance Liquid Chromatography (HPLC) Method for Analysis of Glutathione oxidized (GSSG), S-Nitroso-L-glutathione (GSNO)

Condition

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN -10%
BufferH2SO4 0.2%
Flow Rate1.0 ml/min
DetectionUV 200, 355 nm

Description

Class of CompoundsThiol, Amino acid
Analyzing CompoundsS-Nitroso-L-glutathione (GSNO), Glutathione oxidized (GSSG)

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Glutathione oxidized (GSSG)
S-Nitroso-L-glutathione (GSNO)
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.