HPLC MS Method for Separation of Cyclosporin B on Primesep 200 Column

HPLC Method for Analysis of Cyclosporin B on Primesep NC by SIELC Technologies

HPLC Method for Analysis of Cyclosporin B in on Primesep 200 Column

Cyclosporine B is a calcineurin inhibitor and analogue of cyclosporin A, with the molecular formula C61H109N11O12. It is used primarily as a an immunosuppressant medication with antifungal activities. It is said to work inhibiting P-glycoprotein activity and suppressing the growth of T-lymphoma cell lines. You can find detailed UV spectra of Cyclosporine B and information about its various lambda maxima by visiting the following link.

Cyclosporin B can be retained and analyzed using the Primesep 200 stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of acetonitrile, water, and Ammonium Formate. Detection is performed using UV.

Condition

ColumnPrimesep 200, 3.2 x 150 mm, 3 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O – 50/50%
BufferAmmonium Formate pH 3.0 – 10 mM
Flow Rate0.5 ml/min
DetectionESI-SIM: [M+H]+ m/z 1189
Column Temperature60 C
LOD*55 ppb
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Description

Class of CompoundsDrug
Analyzing CompoundsCyclosporin B

Application Column

Primesep 200

Column Diameter: 3.2 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Cyclosporin B
Application Detection:
LC MS Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.