HPLC Method for Analysis of Phenylephrine HCl on Primesep S Column
Separation type: Liquid Chromatography Mixed-mode
Phenylephrine HCl is a fairly popular decongestant sold under many brand names including Mucinex, Sudafed PE, Sinex, and many other generic brands. While it is best known as a decongestant, it has a wide variety of other pharmaceutical applications, including pupil dilation, hypotension (low blood pressure) treatment, and hemorrhoid relief. Phenylephrine HCl can be detected in the low UV regime. Using a Primesep S normal-phase column and a mobile phase consisting of water and Acetonitrile (MeCN) with a Monosodium Phosphate (NaH2PO4) buffer, Phenylephrine HCl can be retained and analyzed. This analysis method can be UV detected at 220 nm.
| Column | Primesep S, 4.6×100 mm, 3 µm, 100A |
| Mobile Phase | MeCN – 35% |
| Buffer | NaH2PO4 pH 4.5 – 2.6 g/l |
| Flow Rate | 2.0 ml/min |
| Detection | UV 220 nm |
| Retention Time | 4.12 min |
| Class of Compounds | Drug |
| Analyzing Compounds | Phenylephrine |
Application Column
Primesep S
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
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