High Performance Liquid Chromatography (HPLC) Method for Analysis of Isophthalic acid on Primesep B by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Isophthalic acid
Isophthalic acid is an aromatic dicarboxylic acid with the chemical formula C₆H₄(CO₂H)₂. It is a colorless crystalline solid and is one of the three isomers of benzene dicarboxylic acid, the others being phthalic acid and terephthalic acid. In isophthalic acid, the two carboxyl groups (−COOH) are positioned on the 1st and 3rd carbons of the benzene ring, making it a meta isomer.
Uses:
- Polyester production: Isophthalic acid is mainly used in the production of resins like polyethylene terephthalate (PET), which are important in the manufacturing of plastic bottles and fibers.
- Coatings and paints: It is also used in the formulation of alkyd resins for coatings and paints.
- Polyamide resins and epoxy curing agents: Isophthalic acid is used to enhance the chemical resistance and durability of these materials.
Isophthalic acid can be retained, separated and analyzed using a Primesep B mixed-mode stationary phase column. The analysis employs a gradient method with a simple mobile phase comprising water, acetonitrile (MeCN), and phosphoric acid as a buffer. This method allows for detection using UV 210 nm.
You can find detailed UV spectra of Isophthalic acid and information about its various lambda maxima by visiting the following link.
| Column | Primesep B, 4.6 x 150 mm, 5 µm, 100 A |
| Mobile Phase | Gradient MeCN – 30-70%, 10 min |
| Buffer | H3PO4 – 0.4% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 210 nm |
| Limit of Detection | 5 ppb |
| Class of Compounds | Acid |
| Analyzing Compounds | Isophthalic acid |
Application Column
Primesep B
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
