HPLC Method for Analysis of Isophthalic acid on Primesep B Column

High Performance Liquid Chromatography (HPLC) Method for Analysis of Isophthalic acid on Primesep B by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode SIELC Technologies

HPLC Method for Analysis of Isophthalic acid on Primesep B Column


 High Performance Liquid Chromatography (HPLC) Method for Analysis of Isophthalic acid

Isophthalic acid is an aromatic dicarboxylic acid with the chemical formula C₆H₄(CO₂H)₂. It is a colorless crystalline solid and is one of the three isomers of benzene dicarboxylic acid, the others being phthalic acid and terephthalic acid. In isophthalic acid, the two carboxyl groups (−COOH) are positioned on the 1st and 3rd carbons of the benzene ring, making it a meta isomer.

Uses:

  • Polyester production: Isophthalic acid is mainly used in the production of resins like polyethylene terephthalate (PET), which are important in the manufacturing of plastic bottles and fibers.
  • Coatings and paints: It is also used in the formulation of alkyd resins for coatings and paints.
  • Polyamide resins and epoxy curing agents: Isophthalic acid is used to enhance the chemical resistance and durability of these materials.

Isophthalic acid can be retained, separated and analyzed using a Primesep B mixed-mode stationary phase column. The analysis employs a gradient method with a simple mobile phase comprising water, acetonitrile (MeCN), and phosphoric acid as a buffer. This method allows for detection using UV 210 nm.

You can find detailed UV spectra of Isophthalic acid and information about its various lambda maxima by visiting the following link.

ColumnPrimesep B, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseGradient MeCN – 30-70%, 10 min
BufferH3PO4 – 0.4%
Flow Rate1.0 ml/min
DetectionUV 210 nm
Limit of Detection5 ppb
* LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of Compounds
Acid
Analyzing CompoundsIsophthalic acid

Application Column

Primesep B

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Isophthalic acid
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.