HPLC MS Method for Analysis of Sodium dodecyl sulfate on Lipak Column

HPLC Method for Sodium dodecyl sulfate on Lipak by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Sodium dodecyl sulfate.

Sodium dodecyl sulfate (SDS), or sodium lauryl sulfate (SLS), is a surfactant used in cleaning, hygienic, and food products. It has a chemical formula C12H25NaSO4, meaning it has twelve carbon hydrophobic tail attached to a polar sulfate. At low concentrations, it is used as a whipping aid and emulsifier in foods containing egg whites. It has a similar use in pharmaceutics as an ionic solubilizer and, also, an emulsifier. In laboratory uses, it is used as a component for lysing cells during RNA extraction or DNA extraction.

Sodium dodecyl sulfate can be retained and analyzed using the Lipak stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) buffer. Detection is performed using LC MS.

ColumnLipak, 3.2 x 150 mm, 3 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O – 60/40% – 90/10%
BufferAmmonium Formate 10 mM, Formic Acid – 0.05%
Flow Rate0.5 mL/min
DetectionLC MS ESI SIM 265 [M-H] –
LOD*8 ppm
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of CompoundsSurfactant
Analyzing CompoundsSodium dodecyl sulfate

Application Column

Lipak

Column Diameter: 3.2 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Sodium dodecyl sulfate
Application Detection:
LC MS Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.