HPLC Method for Separation of DOTA and DO3A on BIST B+ Column

HPLC Method for Analysis of Tetraxetan (DOTA), DO3A on BIST B+ Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Tetraxetan (DOTA), DO3A.

DOTA is a macrocyclic cheating agent with the chemical formula C16H28N4O8. DOTA is actually an acronym for 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid. It is used to bind metal ions, partially due to it’s stability. It is often employed in biomedical applications including but not limited to MRI contrast agents and targeted cancer therapy. You can find detailed UV spectra of DOTA and information about its various lambda maxima by visiting the following link.

DO3A is a derivative of DOTA with the chemical formula C₁₄H₂₃N₄O₆Na₃. It is primarily used in pre-clinical research as a chelator, which binds radiometals. lanthanides, and manganese.

Tetraxetan (DOTA), DO3A can be retained and analyzed using the BIST B+ stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a phosphoric acid buffer. Detection is performed using UV.

Condition

ColumnBIST B+, 4.6 x 50 mm, 5 µm, 100 A, surface coated
Mobile PhaseMeCN/H2O – 70/30%
BufferH3PO4 – 0.2%
Flow Rate1.0 ml/min
DetectionUV 200
Limit Of Detection*1 – 0.4 ppm, 2- 0.5 ppm
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Description

Class of CompoundsChealting Agent
Analyzing CompoundsTetraxetan (DOTA), DO3A

Application Column

BIST B+

Column Diameter: 4.6 mm
Column Length: 50 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: surface coated

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Application Analytes:
DO3A
Tetraxetan (DOTA)
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.