HPLC–ELSD Method for the Analysis of Methylphosphonic Acid, Glyphosate, and Phosphoric Acid Using a Newcrom BH Column

HPLC Method for Methylphosphonic Acid, Glyphosate, Phosphoric Acid on Newcrom BH by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Methylphosphonic Acid, Glyphosate, Phosphoric Acid.

Methylphosphonic acid is an organophosphorus compound with the chemical formula CH3P(OH)2. It is often used in some lubricant additives, textile treatments, and in synthesis of phosphonate compounds, like Glyphosate.

Glyphosate is an herbicide with a chemical formula of C3H8NO5P. It works through blocking enzymes, like 5-enolpyruvylshikimate-3-phosphate synthase, essential for plant growth. It is typically found in agricultural work, but can occasionally be found in forestry and garden care. You can find detailed UV spectra of Glyphosate and information about its various lambda maxima by visiting the following link.

Phosphoric acid is an inorganic compound with chemical formula H3PO4. It is odorless and colorless, which leads to it’s common use in soft drunks to help preserve the product. It is also used in fertilizers, metal treatment, and corrosion inhibition. Excessive intake of it is not recommended.

Methylphosphonic Acid, Glyphosate, Phosphoric Acid can be retained and analyzed using the Newcrom BH stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a phosphoric acid buffer. Detection is performed using UV.

ColumnNewcrom BH, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O – 40/60%
BufferFormic Acid gradient 0.5% – 2% in 15 min
Flow Rate1.0 mL/min
DetectionELSD, the nebulizer and evaporator temperatures   50°C with a gas flow rate of 1.6 Standard Liters per Minute (SLM)
LOD*1. 0.42 ppm, 2. 0.41 ppm, 3. 0.6 ppm
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of CompoundsHerbicides
Analyzing CompoundsMethylphosphonic Acid, Glyphosate, Phosphoric Acid

Application Column

Newcrom BH

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Glyphosate
Methylphosphonic Acid
Phosphoric Acid
Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.