HPLC UV Method for Simultaneous Analysis of Glyphosate and N-(Phosphonomethyl)iminodiacetic acid (PMIDA) on Newcrom BH  Column

HPLC Method for PMIDA (Phosphonomethyliminodiacetic acid), Glyphosate on Newcrom BH by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of PMIDA (Phosphonomethyliminodiacetic acid), Glyphosate.

Glyphosate is a broad-spectrum herbicide. It works through inhibiting the plant enzyme 5-enolpyruvylshikimate-3-phosphate synthase. The enzyme is essential for producing amino acids within the plant.  Glyphosate is used across agriculture and forestry, as well as rare aquatic environments.  It’s chemical formula is C3H8NO5P. You can find detailed UV spectra of Glyphosate and information about its various lambda maxima by visiting the following link.

N-(Phosphonomethyl)iminodiacetic acid, also known as PMIDA, is a key intermediate in the making of glyphosate. It has the chemical formula (HO)2P(O)CH2N(CH2CO2H)2. You can find detailed UV spectra of PMIDA and information about its various lambda maxima by visiting the following link.

PMIDA (Phosphonomethyliminodiacetic acid), Glyphosate can be retained and analyzed using the Newcrom BH stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a phosphoric acid buffer. Detection is performed using UV.

ColumnNewcrom BH, 4.6 x 50 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O
BufferGradient H3PO4 – 0.1-1%, 10 min
Flow Rate1.0 ml/min
DetectionUV, 200 nm
LOD*4 ppm
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of CompoundsHerbidices
Analyzing CompoundsPMIDA (Phosphonomethyliminodiacetic acid), Glyphosate

Application Column

Newcrom BH

Column Diameter: 4.6 mm
Column Length: 50 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Glyphosate
PMIDA (Phosphonomethyliminodiacetic acid)
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.