HPLC Method for Tirzepatide on Solid-Core Primesep S by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Tirzepatide
Tirzepatide is a synthetic peptide that functions as a dual agonist of the GLP-1 and GIP receptors. It is used in the treatment of type 2 diabetes and obesity. By mimicking the actions of both glucagon-like peptide-1 (GLP-1) and gastric inhibitory polypeptide (GIP), tirzepatide enhances insulin secretion, reduces blood sugar levels, promotes satiety, and aids in weight loss.
Tirzepatide can be retained and analyzed using the Solid-Core Primesep SB stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water, acetonitrile (MeCN), and Ammonium formate. Detection was performed using UV absorption at 280 nm. Mass spectrometric detection was carried out in ESI-SIM mode, monitoring the ions [M+3H]³⁺ at m/z 1606
| Column | Solid-Core Primesep S, 3.2 x 100 mm, 2.7 µm, 90 A |
| Mobile Phase | Gradient MeCN 30-70%, 10 min |
| Buffer | Ammonium Formate pH 3.0 – 40 mM |
| Flow Rate | 0.5 ml/min |
| Detection | UV 280 nm, ESI-SIM: [M+3H]3⁺ -1606 |
| LOD | 8 ppb |
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.
| Class of Compounds | GLP-1 Receptor Agonist |
| Analyzing Compounds | Tirzepatide |
Application Column
Solid-Core Primesep S
Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 2.7 µm
Pore Size: 90 A
UV Detection
