HPLC Method for Analysis of Tyramine on Primesep 100 Column on Alltesta™

HPLC Method for Tyramine on Primesep 100 by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of Tyramine

Tyramine is an amine compound with the molecular formula C8H11NO. As a monoamine oxidase (MAO) substrate, it is broken down by the enzyme MAO in the body. It is typically found in fermented, aged, smoked, and dried foods.

Tyramine can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.

ColumnPrimesep 100, 3.2 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 50%
BufferSulfuric Acid
Flow Rate0.5 ml/min
DetectionUV 275 nm

Class of CompoundsAmine
Analyzing CompoundsTyramine

Application Column

Primesep 100

Column Diameter: 3.2 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Tyramine
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.