HPLC Method for Analysis of trans-Zeatin on Primesep 100 Column on Alltesta™

HPLC Method for trans-Zeatin on Primesep 100 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of trans-Zeatin 

trans-Zeatin is a cytokinin derived from adenine with two forms, cis- and trans-isomer. It has the chemical formula C10H13N5O. It promotes callus initiation and fruit set, delays yellowing for vegetables, and causes auxiliary stems to grow. You can find detailed UV spectra of trans-Zeatin and information about its various lambda maxima by visiting the following link.

trans-Zeatin can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with sulfuric acid as a buffer. Detection is performed using UV.

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 40%
BufferSulfuric Acid
Flow Rate1.0 mL/min
DetectionUV 275 nm
Class of CompoundsCytokinin
Analyzing Compoundstrans-Zeatin

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
trans-Zeatin
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.