HPLC Method for Analysis of Sodium Selenate on Chromni Column

HPLC Method for Analysis of Sodium Selenate on Chromni Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Sodium Selenate.

Sodium Selenate is an inorganic compound with the formula Na2SeO4. It is used primarily as an ingredient in livestock feed multivitamins to increase selenium intake. Selenium produces a red hue in glass, while Sodium Selenate is often used as a decolorizing agent. It iss also a common ingredient in pesticides.

Sodium Selenate can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN). Detection is performed using ELSD.

Condition

ColumnChromni, 4.6 x 100 mm, 3 µm, 100 A, surface coated
Mobile PhaseMeCN/H2O – 80/20
BufferAmAc pH 3.0 – 10 mM
Flow Rate1.0 ml/min
DetectionELSD, the nebulizer and evaporator temperatures
50°C with a gas flow rate of 1.6 Standard Liters per Minute (SLM)
LOD*0.5 ppм
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Description

Class of CompoundsInorganic
Analyzing CompoundsSodium Selenate

Application Column

Chromni

Column Diameter: 4.6 mm
Column Length: 100 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: surface coated

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Application Analytes:
Sodium Selenate
Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.