HPLC Method for Analysis of Pyridoxine on Primesep 100 Column on Alltesta™

HPLC Method for Vitamin B6 (Pyridoxine) on Primesep 100 by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of Vitamin B6 (Pyridoxine)

Pyridoxine, also known as Vitamin B6, with the chemical formula C8H11NO3. It is an essential nutrient required by the body to produce red blood cells and for proper nerve functioning. Sources of it include fish, beef liver, chicken, eggs, dark leafy greens, potatoes, chickpeas, cereals, and more. Deficiency of Vitamin B6 can case dermatitis, sores in the mouth, depression, anemia, and seizures. You can find detailed UV spectra of Vitamin B6 (Pyridoxine) and information about its various lambda maxima by visiting the following link.

Vitamin B6 (Pyridoxine) can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with phosphoric acid as a buffer. Detection is performed using UV.

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 50%
BufferH2SO4 – 0.2%
Flow Rate1.0 ml/min
DetectionUV 275 nm

Class of CompoundsVitamin
Analyzing CompoundsVitamin B6 (Pyridoxine)

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Vitamin B6 (Pyridoxine)
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.