HPLC Method for Analysis of Prochloraz on Newcrom B Column on Alltesta™

HPLC Method for Prochloraz on Newcrom B by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of Prochloraz

Prochloraz is an imidazole fungicide with the chemical formula C15H16Cl3N3O2. It is used widely across Europe, Asia, South America, and Australia, but is not registered for use in the United States. It works as an inhibitor of the enzyme lanosterol 14α-demethylase.

Prochloraz can be retained and analyzed using the Newcrom B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with phosphoric acid as a buffer. Detection is performed using UV.

ColumnNewcrom B, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 50%
BufferAmmonium Formate
Flow Rate1.0 mL/min
DetectionUV 275 nm

Class of CompoundsHydrophobic, Herbicide, Pesticide
Analyzing CompoundsProchloraz

Application Column

Newcrom B

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Prochloraz
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.