HPLC Method for Analysis of Phenazopyridine in Pharmaceutical Formulation on Primesep 100 Column

HPLC Method for Analysis of Phenazopyridine on Primesep 100 Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Phenazopyridine.

Phenazopyridine is an drug with the chemical formula C11H11N5. It is has a local analgesic effect on the urinary tract, therefore it is often used to help with pain, irritation, and urgency caused by urinary tract infections, injury, or surgery. It is not yet known exactly how the drug works.

Phenazopyridine can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN). Detection is performed using UV.

Condition

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O – 70/30
BufferH2SO4 – 0.1%
Flow Rate1.0 ml/min
DetectionUV 390nm
Limit Of Detection*80 ppb
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Description

Class of CompoundsDrug
Analyzing CompoundsPhenazopyridine

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Phenazopyridine
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.