Low-UV Detection HPLC Method for PEI Analysis on PEI Column

HPLC Method for Polyethylenimine PEI on PEI by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of Polyethylenimine PEI

Polyethylenimine (PEI), also known as polyaziridine, is a polymer with the molecular formula (C2H5N)n. It has repeating units of the amine group and carbon aliphatic spacers. PEI has a large variety of uses. Most commonly, it is used in detergents, adhesives, water treatment agents, and cosmetics. Medically, due to being a catatonic polymer, it has been also studied as a gene delivery vehicle with successful outcomes.

Polyethylenimine PEI can be retained and analyzed using the PEI stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN). Detection is performed using UV.

ColumnPEI, 4.6 x 250 mm, 5 µm, 100 A, dual ended
Mobile Phase0.05% HClO4 in MeCN/H2O – 20/80%
Flow Rate0.5 mL/min
DetectionUV 200
LOD* UV60 ppb
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of CompoundsPolymer
Analyzing CompoundsPolyethylenimine PEI

Application Column

PEI

Column Diameter: 4.6 mm
Column Length: 250 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Polyethylenimine PEI
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.