HPLC Method for Analysis of Lycopene on Lipak Column

HPLC Method for Analysis of Lycopene on Lipak Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Lycopene.

Lycopene is a carotenoid hydrocarbon with C40H56 molecular formula. It is found primarily in tomatoes, watermelon, grapefruits, and red guavas. Gac has the highest concentration of lycopene at 2~6 per gram. Lycopene in non-toxic, but can be a rare allergen. You can find detailed UV spectra of Lycopeneand information about its various lambda maxima by visiting the following link.

Lycopene can be retained and analyzed using the Lipak stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) and ethanol. Detection is performed using UV.

Condition

ColumnLipak, 3.2 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN/EtOH – 40%
BufferNone
Flow Rate0.5 ml/min
DetectionVis 427 nm
Limit Of Detection*0.2 ppm
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Description

Class of CompoundsHydrocarbon
Analyzing CompoundsLycopene

Application Column

Lipak

Column Diameter: 3.2 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Lycopene
Application Detection:
Vis Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.