HPLC Method for Analysis of Luminol on Primesep 100 Column

HPLC Method for Analysis of Luminol on Primesep 100 Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Luminol.

Luminol is a compound with the chemical formula C8H7N3O2. It is primarily known for exhibiting chemiluminescence, glowing blue when mixed with an oxidizing agent. It has been used in criminal investigation since the 1930s due to it’s luminescence being enhanced when mixed with blood. Due to the small amount of iron needed as a catalyst, it shows traces of blood even when blood has been cleaned or removed. You can find detailed UV spectra of Luminol and information about its various lambda maxima by visiting the following link.

Luminol can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) and water. Detection is performed using UV.

Condition

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O – 20/80%
BufferH2SO4 – 0.1%
Flow Rate1.0 ml/min
DetectionUV 354 nm
Limit Of Detection*90 ppb
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Description

Class of CompoundsHydrazide
Analyzing CompoundsLuminol

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Luminol
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.