HPLC Chiral Separation of Guaifenesin on Cyralis CM Column

HPLC Method for Analysis of Guaifenesin on Cyralis CM Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Guaifenesin.

Guaifenesin, also known as glyceryl guaiacolate, is a drug with the chemical formula C10H14O4. It is used to remove sputum, or phlegm, from the respiratory tract. It works through stimulating the gastric mucosa, which leads to a higher volume and reduced viscosity of secretions in the trachea and the bronchi. You can find detailed UV spectra of Guaifenesin and information about its various lambda maxima by visiting the following link.

This application describes a normal-phase chiral HPLC method for the separation of Guaifenesin enantiomers using SIELC’s Cyralis CM chiral column. Cyralis CM is a cellulose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications.

Guaifenesin can be retained and analyzed using the Cyralis CM stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of Hexane and Ethanol. Detection is performed using UV.

Condition

ColumnCyralis CM, 4.6 x 100 mm, 5 µm, 100 A, dual ended
Mobile PhaseHexane/Ethanol- 50/40%
Flow Rate1.0 mL/min
DetectionUV 230 nm
Resolution6.04

Description

Class of CompoundsDrug
Analyzing CompoundsGuaifenesin

Application Column

Cyralis CM

Column Diameter: 4.6 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Guaifenesin
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.