HPLC Chiral Separation of Fluroxypyr-Meptyl on Cyralis CM Column

HPLC Method for Analysis of Fluroxypyr-meptyl on Cyralis CM Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Fluroxypyr-meptyl.

Fluroxypyr-Meptyl is a herbicide with the chemical formula C15H21Cl2FN2O3. It is used for the control of broad-leaved weeds in cereals, pastures, and non-crop areas. It belongs to a class of herbicides known as synthetic auxins. Fluroxypyr-meptyl is effective against a range of weed species, including some that are resistant to other types of herbicides. You can find detailed UV spectra of Fluroxypyr-meptyl and information about its various lambda maxima by visiting the following link.

This application describes a normal-phase chiral HPLC method for the separation of Fluroxypyr-meptyl enantiomers using SIELC’s Cyralis CM chiral column. Cyralis CM is a cellulose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications.

Fluroxypyr-meptyl can be retained and analyzed using the Cyralis CM stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of Hexane, IPA, and DEA. Detection is performed using UV.

Condition

ColumnCyralis CM, 4.6 x 250 mm, 5 µm, 100 A, dual ended
Mobile PhaseHexane/IPA/DEA – 85/15/0.5%
Flow Rate1.0 mL/min
DetectionUV 250 nm
Resolution1.89

Description

Class of CompoundsHerbicide
Analyzing CompoundsFluroxypyr-meptyl

Application Column

Cyralis CM

Column Diameter: 4.6 mm
Column Length: 250 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Fluroxypyr-meptyl
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.