HPLC Method for Analysis of Diphenylamine on Newcrom R1 Column on Alltesta™

HPLC Method for Diphenylamine on Newcrom R1 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Diphenylamine 

Diphenylamine is an organic compound with the chemical formula (C6H5)2NH. It is used primarily to distinguish DNA from RNA with the Dsiche test, as a scald inhibitor in apples, antioxidant, stabilizer of smokeless powder, and a redox indicator. It’s a derivative of aniline, while Metanil Yellow, Disperse Orange 1, and Acid Orange 5 are derivatives of Diphenylamine. You can find detailed UV spectra of Diphenylamine and information about its various lambda maxima by visiting the following link.

Diphenylamine can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with phosphoric acid as a buffer. Detection is performed using UV.

ColumnNewcrom R1, 3.2 x 100 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN -60/40%
BufferH3PO4 – 0.1%
Flow Rate0.5 mL/min
DetectionUV 275 nm
Class of CompoundsOrganic Compound
Analyzing CompoundsDiphenylamine

Application Column

Newcrom R1

Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Diphenylamine
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.